LOCUS       sequence-280418-        1376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1227
FEATURES             Location/Qualifiers
     source          1..1376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccctccc gacaccctcg gacagcaccc tccccgcgga agcccgggga
       61 cgaggacggc gacggagact cgtttggacc ccgagccaaa gcgaggccct gcgagcctgc
      121 tttgagcgga acccgtaccc gggcatcgcc accagagaac ggctggccca ggccatcggc
      181 attccggagc ccagggtcca gatttggttt cagaatgaga ggtcacgcca gctgaggcag
      241 caccggcggg aatctcggcc ctggcccggg agacgcggcc cgccagaagg ccggcgaaag
      301 cggaccgccg tcaccggatc ccagaccgcc ctgctcctcc gagcctttga gaaggatcgc
      361 tttccaggca tcgccgcccg ggaggagctg gccagagaga cgggcctccc ggagtccagg
      421 attcagatct ggtttcagaa tcgaagggcc aggcacccgg gacagggtgg cagggcgccc
      481 gcgcaggcag gcggcctgtg cagcgcggcc cccggcgggg gtcaccctgc tccctcgtgg
      541 gtcgccttcg cccacaccgg cgcgtgggga acggggcttc ccgcacccca cgtgccctgc
      601 gcgcctgggg ctctcccaca gggggctttc gtgagccagg cagcgagggc cgcccccgcg
      661 ctgcagccca gccaggccgc gccggcagag gggatctccc aacctgcccc ggcgcgcggg
      721 gatttcgcct acgccgcccc ggctcctccg gacggggcgc tctcccaccc tcaggctcct
      781 cggtggcctc cgcacccggg caaaagccgg gaggaccggg acccgcagcg cgacggcctg
      841 ccgggcccct gcgcggtggc acagcctggg cccgctcaag cggggccgca gggccaaggg
      901 gtgcttgcgc cacccacgtc ccaggggagt ccgtggtggg gctggggccg gggtccccag
      961 gtcgccgggg cggcgtggga accccaagcc ggggcagctc cacctcccca gcccgcgccc
     1021 ccggacgcct ccgcctccgc gcggcagggg cagatgcaag gcatcccggc gccctcccag
     1081 gcgctccagg agccggcgcc ctggtctgca ctcccctgcg gcctgctgct ggatgagctc
     1141 ctggcgagcc cggagtttct gcagcaggcg caacctctcc tagaaacgga ggccccgggg
     1201 gagctggagg cctcggaaga ggccgcctcg ctggaagcac ccctcagcga ggaagaatac
     1261 cgggctctgc tggaggagct ttagtaatga actagtccgt aacttgaaag tatttcgatt
     1321 tcttggcttt atatatcttg tggaaaggac gactgctgag ctgggcacat gcccta
//
