LOCUS       sequence-280424-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1240
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaaaaag gacttcccta gaagtgagtc ttggggagtt ggggggagaa
       61 aagtgtcgag gagggcgtcg gagtttccca ccgctggctg cttcccggcc cgcacgcccg
      121 ggagggtggc ggtgggcgcg cagagatctt tgcaaaacag cgtccagggc ggaaaacaac
      181 tcacaggcct gccgccccca aaggcgggca ggtccggacg cgctgggccc tggtcccttc
      241 ggccgcaaac ggcgcaagtc acgcactgcg ttcaccgcgc aacaggtgct ggagctggag
      301 cggcgcttcg tcttccagaa gtacctggcg ccgtccgagc gagacgggct agctacgcga
      361 ctcggcctgg ccaacgcgca ggtggtcact tggttccaga accggcgagc caagctcaag
      421 cgcgatgtgg aggagatgcg cgccgacgtc gcctcgctac gcgcgttgtc cccggaagtc
      481 ctgtgcagct tagcactgcc cgaaggcgct ccagatcccg gcctctgcct cggccctgcc
      541 ggccctgact cccggcccca cctgtcagac gaggagatac aggtggacga ttagtaatga
      601 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      661 gaagtgaccc tcctcgttga tttgca
//
