LOCUS       sequence-280433-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1254
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccatcta caagcagtca cagcacatga cggaggttgt gaggcgctgc
       61 ccccaccatg agcgctgctc agatagcgat ggtctggccc ctcctcagca tcttatccga
      121 gtggaaggaa atttgcgtgt ggagtatttg gatgacagaa acacttttcg acatagtgtg
      181 gtggtgccct atgagccgcc tgaggttggc tctgactgta ccaccatcca ctacaactac
      241 atgtgtaaca gttcctgcat gggcggcatg aaccggaggc ccatcctcac catcatcaca
      301 ctggaagact ccagtggtaa tctactggga cggaacagct ttgaggtgcg tgtttgtgcc
      361 tgtcctggga gagaccggcg cacagaggaa gagaatctcc gcaagaaagg ggagcctcac
      421 cacgagctgc ccccagggag cactaagcga gcactgccca acaacaccag ctcctctccc
      481 cagccaaaga agaaaccact ggatggagaa tatttcaccc ttcagatccg tgggcgtgag
      541 cgcttcgaga tgttccgaga gctgaatgag gccttggaac tcaaggatgc ccaggctggg
      601 aaggagccag gggggagcag ggctcactcc agccacctga agtccaaaaa gggtcagtct
      661 acctcccgcc ataaaaaact catgttcaag acagaagggc ctgactcaga ctagtaatga
      721 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      781 gagcatacta gctatcagaa ctcttc
//
