LOCUS       sequence-280442-        1283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1257
FEATURES             Location/Qualifiers
     source          1..1283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..1191
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
ORIGIN
        1 accgccacca tggaggagcc gcagtcagat cctagcgtcg agccccctct gagtcaggaa
       61 acattttcag acctatggaa actacttcct gaaaacaacg ttctgtcccc cttgccgtcc
      121 caagcaatgg atgatttgat gctgtccccg gacgatattg aacaatggtt cactgaagac
      181 ccaggtccag atgaagctcc cagaatgcca gaggctgctc cccccgtggc ccctgcacca
      241 gcagctccta caccggcggc ccctgcacca gccccctcct ggcccctgtc atcttctgtc
      301 ccttcccaga aaacctacca gggcagctac ggtttccgtc tgggcttctt gcattctggg
      361 acagccaagt ctgtgacttg cacgtactcc cctgccctca acaagatgtt ttgccaactg
      421 gccaagacct gccctgtgca gctgtgggtt gattccacac ccccgcccgg cacccgcgtc
      481 cgcgccatgg ccatctacaa gcagtcacag cacatgacgg aggttgtgag gcgctgcccc
      541 caccatgagc gctgctcaga tagcgatggt ctggcccctc ctcagcatct tatccgagtg
      601 gaaggaaatt tgcgtgtgga gtatttggat gacagaaaca cttttcgaca tagtgtggtg
      661 gtgccctatg agccgcctga ggttggctct gactgtacca ccatccacta caactacatg
      721 tgtaacagtt cctgcatggg cggcatgaac cggaggccca tcctcaccat catcacactg
      781 gaagactcca gtggtaatct actgggacgg aacagctttg aggtgcgtgt ttgtgcctgt
      841 cctgggagag accggcgcac agaggaagag aatctccgca agaaagggga gcctcaccac
      901 gagctgcccc cagggagcac taagcgagca ctgcccaaca acaccagctc ctctccccag
      961 ccaaagaaga aaccactgga tggagaatat ttcacccttc agatccgtgg gcgtgagcgc
     1021 ttcgagatgt tccgagagct gaatgaggcc ttggaactca aggatgccca ggctgggaag
     1081 gagccagggg ggagcagggc tcactccagc cacctgaagt ccaaaaaggg tcagtctacc
     1141 tcccgccata aaaaactcat gttcaagaca gaagggcctg actcagacta gtaatgaact
     1201 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1261 cgctctacag agactgtatc ggc
//
