LOCUS       sequence-280445-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1258
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgattt gatgctgtcc ccggacgata ttgaacaatg gttcactgaa
       61 gacccaggtc cagatgaagc tcccagaatg ccagaggctg ctccccccgt ggcccctgca
      121 ccagcagctc ctacaccggc ggcccctgca ccagccccct cctggcccct gtcatcttct
      181 gtcccttccc agaaaaccta ccagggcagc tacggtttcc gtctgggctt cttgcattct
      241 gggacagcca agtctgtgac ttgcacgtac tcccctgccc tcaacaagat gttttgccaa
      301 ctggccaaga cctgccctgt gcagctgtgg gttgattcca cacccccgcc cggcacccgc
      361 gtccgcgcca tggccatcta caagcagtca cagcacatga cggaggttgt gaggcgctgc
      421 ccccaccatg agcgctgctc agatagcgat ggtctggccc ctcctcagca tcttatccga
      481 gtggaaggaa atttgcgtgt ggagtatttg gatgacagaa acacttttcg acatagtgtg
      541 gtggtgccct atgagccgcc tgaggttggc tctgactgta ccaccatcca ctacaactac
      601 atgtgtaaca gttcctgcat gggcggcatg aaccggaggc ccatcctcac catcatcaca
      661 ctggaagact ccagtggtaa tctactggga cggaacagct ttgaggtgcg tgtttgtgcc
      721 tgtcctggga gagaccggcg cacagaggaa gagaatctcc gcaagaaagg ggagcctcac
      781 cacgagctgc ccccagggag cactaagcga gcactgccca acaacaccag ctcctctccc
      841 cagccaaaga agaaaccact ggatggagaa tatttcaccc ttcagatgct acttgactta
      901 cgatggtgtt acttcctgat aaactcgtcg tagtaatgaa ctagtccgta acttgaaagt
      961 atttcgattt cttggcttta tatatcttgt ggaaaggacg attatccgga ggaggatgat
     1021 cgcaa
//
