LOCUS       sequence-280460-        1319 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1273
FEATURES             Location/Qualifiers
     source          1..1319
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggctgt ggtggagaat gtagtgaagc tccttgggga gcagtactac
       61 aaagatgcca tggagcagtg ccacaattac aatgctcgcc tctgtgctga gcgcagcgtg
      121 cgcctgcctt tcttggactc acagaccgga gtagcccaga gcaattgtta catctggatg
      181 gaaaagcgac accggggtcc aggattggcc tccggacagc tgtactccta ccctgcccgg
      241 cgctggcgga aaaagcggcg agcccatccc cctgaggatc cacgactttc cttcccatct
      301 attaagccag acacagacca gaccctgaag aaggaggggc tgatctctca ggatggcagt
      361 agtttagagg ctctgttgcg cactgacccc ctggagaagc gaggtgcccc ggatccccga
      421 gttgatgatg acagcctggg cgagtttcct gtgaccaaca gtcgagcgcg aaagcggatc
      481 ctagaaccag atgacttcct ggatgacctc gatgatgaag actatgaaga agatactccc
      541 aagcgtcggg gaaaggggaa atccaagggt aagggtgtgg gcagtgcccg taagaagctg
      601 gatgcttcca tcctggagga ccgggataag ccctatgcct gtgacaatag tttcaaacaa
      661 aagcatacct cgaaagcgcc ccagagagtt tgtggaaaac gttacaagaa ccgaccaggc
      721 ctcagttacc actatgccca ctcccacttg gctgaggagg agggcgagga caaggaagac
      781 tctcaaccac ccactcctgt ttcccagagg tctgaggagc agaaatccaa aaagggtcct
      841 gatggattgg ccttgcccaa caactactgt gacttctgcc tgggggactc aaagattaac
      901 aagaagacgg gacaacccga ggagctggtg tcctgttctg actgtggccg ctcagggcat
      961 ccatcttgcc tccaatttac ccccgtgatg atggcggcag tgaagacata ccgctggcag
     1021 tgcatcgagt gcaaatgttg caatatctgc ggcacctccg agaatgacga ccagttgctc
     1081 ttctgtgatg actgcgatcg tggctaccac atgtactgtc tcaccccgtc catgtctgag
     1141 ccccctgaag gaagttggag ctgccacctg tgtctggacc tgttgaaaga gaaagcttcc
     1201 atctaccaga accagaactc ctcttagtaa tgaactagtc cgtaacttga aagtatttcg
     1261 atttcttggc tttatatatc ttgtggaaag gacgaacaaa ccgttaaatg tggacgtgg
//
