LOCUS       sequence-280463-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1274
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtaaaagc aatccaggtt ttgcgcattc atcttcttga gctggaaaag
       61 gttaacgaac tctgcaaaga tttctgcagt cgatacattg cttgtctgaa aacaaaaatg
      121 aacagtgaaa ctctgttgag tggagagcct ggaagcccgt actcaccagt gcagtcccag
      181 cagattcaaa gtgccatcac aggcaccatc agccctcagg gaattgtggt gccggcgtcc
      241 gcgctgcagc agggaaacgt agccatggcg acggtggcag gtggcacagt gtatcagcct
      301 gtcacggtcg tcactcccca aggccaagtg gtcacacaga cattgtcgcc tgggacaatt
      361 aggatccaga actcccagct tcagttacag ttaaaccaag atctcagcat cttgcatcaa
      421 gatgatggtt catctaagaa caagaggggc gtcctgccaa agcatgccac gaacgtgatg
      481 cggtcctggc tcttccagca catcgggcat ccctacccaa cagaggatga gaaaaaacag
      541 attgctgctc agacaaattt gacactactc caagtcaaca actggttcat caatgccaga
      601 agacgaattc ttcagccaat gttggattca agttgttcag agacccccaa aacaaagaaa
      661 aaaactgctc agaaccggcc agttcagagg ttttggcctg attctattgc atcaggagtc
      721 gcacagccac cgccgagcga gctcaccatg tcggaaggag ctgttgtcac catcaccacg
      781 cccgtgaaca tgaacgtgga cagccttcag tctctgtcct cggacggggc caccctggcg
      841 gtgcagcagg tcatgatggc agggcagagc gaggacgagt ctgtggacag cacagaggag
      901 gatgcgggtg ccctggcccc tgcccacatc agcgggctgg tcttggagaa cagtgactcc
      961 ctgcagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1021 tcttgtggaa aggacgattc cgtcctttcg aattgagtga a
//
