LOCUS       sequence-280484-        1145 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1301
FEATURES             Location/Qualifiers
     source          1..1145
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaaactt gatgactagc tccaccctac cgcccctttt tgcagatgaa
       61 gacggttcca aggagagtaa tgatctggct accactgggt taaatcaccc agaggttcca
      121 tacagtagtg gcgccacatc atccaccaac aatccagaat ttgtggagga tctctctcaa
      181 ggtcagttgc ttcagagtga gtcttcaaat gcagcagaag gcaatgaaca gaggcatgaa
      241 gatgagcaac gaagtaaacg aggaggttgg tccaaaggaa gaaagaggaa gaaacctctt
      301 cgagacagca atgcacccaa atcccccctt acaggatatg ttcggttcat gaatgagcgt
      361 cgagaacaac ttcgagcaaa gagaccagaa gtcccatttc cagaaatcac aaggatgtta
      421 ggcaatgaat ggagtaaact gcctcctgag gaaaaacagc gctaccttga tgaagcagac
      481 agagataagg agcgttacat gaaggaactg gaacagtatc agaaaacaga ggcctacaag
      541 gtcttcagta ggaaaaccca ggaccgtcag aaaggcaaat ctcataggca agatgcagcc
      601 cggcaggcca ctcatgatca tgagaaagaa acagaggtaa aggaacggtc tgtttttgac
      661 atccctatat ttacagagga attcttgaac catagcaaag ctcgggaagc agagctccgc
      721 cagcttcgca aatccaacat ggagtttgag gagaggaatg cagccctgca aaagcacgtg
      781 gagagcatgc gcacagcagt ggagaagctg gaggtggatg tgatccagga gcggagccgc
      841 aacacagtct tacagcagca cctggagacc ctgcggcagg tgctgaccag cagctttgcc
      901 agcatgccct tgcctggaag tggagagaca cctacagtgg acaccattga ctcatatatg
      961 aacagactgc acagtattat tttagctaat ccccaagaca atgaaaactt catagctaca
     1021 gttcgagaag ttgtgaacag actcgatcgt tagtaatgaa ctagtccgta acttgaaagt
     1081 atttcgattt cttggcttta tatatcttgt ggaaaggacg aatatgtata ccgatcgccg
     1141 tggaa
//
