LOCUS       sequence-280496-        1433 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1335
FEATURES             Location/Qualifiers
     source          1..1433
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgaccgc agcgtctaac cactacagcc tgctcacctc cagcgcctcc
       61 atcgtgcacg ccgagccgcc cggcggcatg cagcagggcg cggggggcta ccgcgaagcg
      121 cagagcctgg tgcagggcga ctacggcgct ctgcagagca acggacaccc gctcagccac
      181 gctcaccagt ggatcaccgc gctgtcccac ggcggcggcg gcgggggcgg tggcggcggc
      241 ggggggggcg ggggcggcgg cgggggcggc ggcgacggct ccccgtggtc caccagcccc
      301 ctgggccagc cggacatcaa gccctcggtg gtggtgcagc agggcggccg cggagacgag
      361 ctgcacgggc caggcgccct gcagcagcag catcagcagc agcaacagca acagcagcag
      421 caacagcagc aacagcagca gcagcagcag caacagcggc cgccgcatct ggtgcaccac
      481 gccgctaacc accacccggg acccggggca tggcggagcg cggcggctgc agcgcacctc
      541 ccaccctcca tgggagcgtc caacggcggc ttgctctact cgcagcccag cttcacggtg
      601 aacggcatgc tgggcgccgg cgggcagccg gccggtctgc accaccacgg cctgcgggac
      661 gcgcacgacg agccacacca tgccgaccac cacccgcacc cgcactcgca cccacaccag
      721 cagccgccgc ccccgccgcc cccgcagggt ccgcctggcc acccaggcgc gcaccacgac
      781 ccgcactcgg acgaggacac gccgacctcg gacgacctgg agcagttcgc caagcagttc
      841 aagcagcggc ggatcaaact gggatttacc caagcggacg tggggctggc tctgggcacc
      901 ctgtatggca acgtgttctc gcagaccacc atctgcaggt ttgaggccct gcagctgagc
      961 ttcaagaaca tgtgcaagct gaagcctttg ttgaacaagt ggttggagga ggcggactcg
     1021 tcctcgggca gccccacgag catagacaag atcgcagcgc aagggcgcaa gcggaaaaag
     1081 cggacctcca tcgaggtgag cgtcaagggg gctctggaga gccatttcct caaatgcccc
     1141 aagccctcgg cccaggagat cacctccctc gcggacagct tacagctgga gaaggaggtg
     1201 gtgagagttt ggttttgtaa caggagacag aaagagaaaa ggatgacccc tcccggaggg
     1261 actctgccgg gcgccgagga tgtgtacggg gggagtaggg acactccacc acaccacggg
     1321 gtgcagacgc ccgtccagta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1381 tggctttata tatcttgtgg aaaggacgaa cgaattaata tgtgaacaga ttc
//
