LOCUS       sequence-280499-        1961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1338
FEATURES             Location/Qualifiers
     source          1..1961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccaatga cattgatgag ctcattggca ttcccttccc caaccacagc
       61 agtgaggtcc tgtgcagcct caatgagcaa cggcacgatg gcctgctgtg tgacgtgctc
      121 ctggtggtgc aggagcagga gtatcggacc caccgctccg tcctggctgc ctgcagcaag
      181 tacttcaaga agcttttcac agccggcacc ctagccagcc agccctacgt ctatgagatc
      241 gactttgtcc agcctgaggc tctggctgct atcctggagt tcgcctacac ctccacgctc
      301 accatcaccg ctggcaatgt caagcacatc ctcaacgcag ccaggatgct ggagatccag
      361 tgcatcgtga acgtgtgcct ggagatcatg gagcctgggg gggacggggg ggaggaggat
      421 gacaaggagg acgatgacga cgacgaagat gatgatgatg aggaggacga agaggaggag
      481 gaggaagagg aggaggatga cgatgatgac acggaggact ttgctgacca agaaaacttg
      541 cctgaccccc aggacatcag ctgccaccaa agcccttcca agacagacca tctcacagag
      601 aaggcctatt cagacacccc cagggacttc cctgactcct tccaggctgg cagtcctggc
      661 catctggggg tgatccggga cttctccatc gaatctctgc taagggagaa cctgtacccc
      721 aaggccaaca tccccgacag gagaccctcc ttgtctccat tcgccccgga cttctttcca
      781 cacctctggc caggggactt cggtgccttt gcccagctgc ctgagcagcc catggacagt
      841 gggccactgg atctggtcat caagaatcgg aagatcaagg aggaggagaa ggaggagctg
      901 cccccacccc caccgccacc cttccctaat gacttcttca aggacatgtt ccctgacctg
      961 ccgggggggc ctctgggacc catcaaggcg gagaacgact acggtgccta tctcaacttc
     1021 ctgagtgcca cccacctggg aggcctcttc ccaccctggc ccctggtaga agagcgcaag
     1081 ctgaagccca aggcctctca gcagtgcccc atctgccaca aagtcatcat gggggccggg
     1141 aagctgccgc ggcacatgag gacccatacc ggggagaagc catacatgtg caccatctgc
     1201 gaggtccgct tcaccaggca ggacaagctg aaaatccaca tgcggaagca cacaggggag
     1261 cggccctacc tgtgcatcca ctgcaacgcc aagttcgtgc acaactacga cctcaagaac
     1321 cacatgcgca tccacacggg cgtgcggccc taccagtgcg agttctgcta caagagcttc
     1381 acgcgctctg accacctgca ccgccacatc aagcgccaga gctgccgcat ggcacggccc
     1441 cgacgcggcc gcaagcctgc tgcgtggagg gccgccagcc tgctcttcgg gcccggcggc
     1501 ccggcccccg acaaggcggc cttcgtgatg ccccctgcgc tgggcgaggt gggcggccac
     1561 ctgggcggcg cagctgtgtg cctcccgggc cccagccccg ccaagcactt cctggcagcg
     1621 cccaagggcg ccctgagcct gcaagagctg gagcggcagt tcgaggagac acagatgaag
     1681 ctgttcgggc gcgcgcagct ggaggctgag aggaacgcgg ggggcctcct ggccttcgcg
     1741 ctggccgaga acgtggcggc ggcgcggccc tacttcccgc tgcccgaccc ttgggccgcc
     1801 ggcctggccg gcctccctgg gctcgccggc ctcaaccacg tggcctccat gtccgaagcc
     1861 aacaactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1921 tcttgtggaa aggacgaccg gcgatttcta cacttggtta c
//
