LOCUS       sequence-280502-        1952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1345
FEATURES             Location/Qualifiers
     source          1..1952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtcggcca actgagcgag ggggccattg cggccatcat gcagaagggg
       61 gatacaaaca taaagcccat cctccaagtc atcaacatcc gtcccattac tacggggaat
      121 agtccgccgc gttatcgact gctcatgagt gatggattga acactctatc ctctttcatg
      181 ttggcgacac agttgaaccc tctcgtggag gaagaacaat tgtccagcaa ctgtgtatgc
      241 cagattcaca gatttattgt gaacactctg aaagacggaa ggagagtagt tatcttgatg
      301 gaattagaag ttttgaagtc agctgaagca gttggagtga agattggcaa tccagtgccc
      361 tataatgaag gactcgggca gccgcaagta gctcctccag cgccagcagc cagcccagca
      421 gcaagcagca ggccccagcc gcagaatgga agctcgggaa tgggttctac tgtttctaag
      481 gcttatggtg cttcaaagac atttggaaaa gctgcaggtc ccagcctgtc acacacttct
      541 gggggaacac agtccaaagt ggtgcccatt gccagcctca ctccttacca gtccaagtgg
      601 accatttgtg ctcgtgttac caacaaaagt cagatccgta cctggagcaa ctcccgaggg
      661 gaagggaagc ttttctccct agaactggtt gacgaaagtg gtgaaatccg agctacagct
      721 ttcaatgagc aagtggacaa gttctttcct cttattgaag tgaacaaggt gtattatttc
      781 tcgaaaggca ccctgaagat tgctaacaag cagttcacag ctgttaaaaa tgactacgag
      841 atgaccttca ataacgagac ttccgtcatg ccctgtgagg acgaccatca tttacctacg
      901 gttcagtttg atttcacggg gattgatgac ctcgagaaca agtcgaaaga ctcacttgta
      961 gacatcatcg ggatctgcaa gagctatgaa gacgccacta aaatcacagt gaggtctaac
     1021 aacagagaag ttgccaagag gaatatctac ttgatggaca catccgggaa ggtggtgact
     1081 gctacactgt ggggggaaga tgctgataaa tttgatggtt ctagacagcc cgtgttggct
     1141 atcaaaggag cccgagtctc tgatttcggt ggacggagcc tctccgtgct gtcttcaagc
     1201 actatcattg cgaatcctga catcccagag gcctataagc ttcgtggatg gtttgacgca
     1261 gaaggacaag ccttagatgg tgtttccatc tctgatctaa agagcggcgg agtcggaggg
     1321 agtaacacca actggaaaac cttgtatgag gtcaaatccg agaacctggg ccaaggcgac
     1381 aagccggact actttagttc tgtggccaca gtggtgtatc ttcgcaaaga gaactgcatg
     1441 taccaagcct gcccgactca ggactgcaat aagaaagtga ttgatcaaca gaatggattg
     1501 taccgctgtg agaagtgcga caccgaattt cccaatttca agtaccgcat gatcctgtca
     1561 gtaaatattg cagattttca agagaatcag tgggtgactt gtttccagga gtctgctgaa
     1621 gctatccttg gacaaaatgc tgcttatctt ggggaattaa aagacaagaa tgaacaggca
     1681 tttgaagaag ttttccagaa tgccaacttc cgatctttca tattcagagt cagggtcaaa
     1741 gtggagacct acaacgacga gtctcgaatt aaggccactg tgatggacgt gaagcccgtg
     1801 gactacagag agtatggccg aaggctggtc atgagcatca ggagaagtgc attgatgtag
     1861 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1921 aaggacgagc gaatgaaccg attagtcatt cg
//
