LOCUS       sequence-280505-        4049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1351
FEATURES             Location/Qualifiers
     source          1..4049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggacac cgacctgttc atggaatgtg aggaggagga gttggagcca
       61 tggcagaaaa tcagtgatgt cattgaggac tctgtagttg aagattataa ttcagtggat
      121 aaaactacca cagtttctgt gagccagcag ccagtctcgg ctccagtgcc catcgctgcc
      181 catgcttctg ttgctgggca cctctctaca tccaccaccg ttagtagcag cggggcacag
      241 aacagcgaca gtacaaagaa gactcttgtc acactaattg ccaacaacaa tgccccaggt
      301 acccagtttg ttaagccgac agttggagtt ccacaagtgt tctcccagat gacccctgtg
      361 aggccaggct ccacaatgcc tgtgaggccc accaccaaca ccttcaccac cgtcatcccg
      421 gccactctta ccattcgaag caccgtccca cagtcccagt cccagcagac caagtccact
      481 cccagcactt ctaccactcc cactgccaca cagccaacct cactggggca actagctgtt
      541 cagtctccag gccagtcaaa ccagaccacg aatcccaagc tagctccctc cttcccctct
      601 ccacctgcag tgagcattgc cagctttgtc actgtgaagc gacctggtgt tacaggcgaa
      661 aatagcaatg aagtggccaa attggtgaat acccttaaca ccatcccttc cctgggccag
      721 agtcctgggc cagtggtggt gtccaacaac agctctgctc atggctctca aagaaccagc
      781 ggacctgagt cttcaatgaa agtgacctct tccatcccag tatttgacct ccaggatggt
      841 ggacggaaaa tatgtccacg atgtaatgct caatttcgtg ttactgaagc tttgagaggt
      901 cacatgtgtt actgttgccc agaaatggtt gaataccaga agaaaggaaa gtccctggat
      961 tcagaaccca gtgtcccatc agcagcaaag cccccatccc ctgagaaaac agctcctgtt
     1021 gcttccacac cctcttctac acctattcct gctctgtcac cgcctaccaa agtaccagaa
     1081 ccaaatgaga acgtgggcga tgccgtccag accaaactca ttatgcttgt agatgacttc
     1141 tactatggac gggatggtgg caaagtagcc cagctcacaa atttccctaa ggtcgccaca
     1201 tctttccgat gcccacattg taccaaaagg ctaaaaaaca atattcgatt catgaaccat
     1261 atgaaacacc acgtagaact cgatcagcag aacggtgagg tagatggtca cactatctgc
     1321 cagcactgtt accgccagtt ttccactccc ttccagcttc agtgccactt ggaaaatgtt
     1381 catagtccct atgaatctac taccaagtgc aagatctgtg aatgggcgtt tgaaagtgag
     1441 ccactatttc tccagcatat gaaggatact cataagcctg gagagatgcc ttatgtttgc
     1501 caggtgtgtc aatatcgctc ctcactctac tctgaggtag atgtccattt tcggatgatc
     1561 catgaggata cccggcatct gctctgccct tattgcctga aggtcttcaa aaatggcaat
     1621 gcattccaac agcattacat gaggcaccag aagagaaatg tttatcactg caacaaatgc
     1681 cggctgcagt ttctctttgc caaggacaaa attgaacaca agcttcaaca ccataaaacc
     1741 ttccgtaaac ccaagcagct ggagggcttg aaaccaggca ccaaggtgac aatccgggct
     1801 tcccgagggc agccacgaac tgttcctgta tcctctaatg atacacctcc cagcgccttg
     1861 caggaggcag caccgctgac ctcctcaatg gaccctctgc ctgtcttcct ttatccccct
     1921 gtccagcgca gcatccagaa gagagctgtt aggaaaatga gtgtcatggg ccggcagaca
     1981 tgcctggagt gcagcttcga gatcccagac ttccctaatc atttccctac ttacgtacac
     2041 tgctctctgt gtcgctatag cacctgctgt tctcgagctt atgccaacca catgatcaac
     2101 aatcatgttc cacggaagag ccccaagtat ttggctttgt ttaaaaattc tgtgagtgga
     2161 atcaagctgg cctgcacttc atgtaccttt gttacctctg tgggcgatgc tatggccaag
     2221 catttggtat tcaacccctc tcacagatcc agcagcatcc tgccacgggg actcacttgg
     2281 atagctcact caaggcatgg ccagactcgt gaccgagtgc atgaccggaa cgtgaagaat
     2341 atgtaccctc ctccttcctt ccccactaac aaagctgcca ctgtgaaatc tgcgggggcc
     2401 accccagctg agcctgaaga gctactaact cccttagccc cagcactccc atcaccagcc
     2461 tcaactgcaa ccccaccacc aacccccact cacccgcagg ctttagccct tccaccgctg
     2521 gctacagagg gagccgaatg tctgaatgtt gatgatcagg atgaagggag cccagtcacc
     2581 caagaacctg agctagcatc aggtggtggt ggtagtggtg gagttggcaa aaaggagcag
     2641 ctgtctgtga agaagcttcg agtagtactg tttgctctat gctgcaatac agaacaggca
     2701 gctgaacact tccgaaatcc ccagcgacgt attcgccgtt ggcttcgacg tttccaggcc
     2761 tcccaggggg agaatctaga gggcaaatat ctgagctttg aggcagaaga gaaactggct
     2821 gagtgggtgc taacccagcg cgaacaacag ctacctgtaa atgaggagac cttgttccag
     2881 aaggccacca aaataggacg ttctttggaa ggggggttta agatctccta tgagtgggct
     2941 gtgcgtttca tgctgcggca ccacctgact ccccatgccc ggcgagctgt ggcccacacc
     3001 ctacctaagg atgtagcaga gaatgcagga ctcttcattg attttgtaca acggcagatt
     3061 cacaaccagg acttaccctt gtctatgatt gtggctattg atgagatctc tttgttcctg
     3121 gatacagagg tgctgagcag tgatgatcga aaggagaatg ccctgcagac agtgggcaca
     3181 ggggaacctt ggtgtgatgt agtcctagcc attctggcag atggcactgt ccttcccacc
     3241 ctggttttct acagagggca gatggatcag cctgctaaca tgccagactc catattgcta
     3301 gaggcaaagg agagtggcta cagtgatgac gagatcatgg agctgtggtc aactcgagtg
     3361 tggcagaagc acacagcttg ccagcgcagc aaaggcatgc ttgtgatgga ctgtcatcgc
     3421 actcacttgt cagaagaggt actggctatg cttagtgcct ctagcacttt gcctgcagtg
     3481 gtcccagcag gctgtagctc caaaattcag ccattagatg tatgcatcaa aagaactgtc
     3541 aagaacttcc tgcataaaaa atggaaggaa caggctcggg aaatggcaga tactgcatgt
     3601 gattctgatg tcctgcttca gctggtgctt gtctggctgg gtgaagtgct aggtgtcatt
     3661 ggggactgtc cagagctagt tcagcgctcc ttcctggtgg ctagtgttct gcctggcccc
     3721 gatggcaaca ttaactcacc tacaagaaat gctgacatgc aggaggagct aattgcctcc
     3781 ctagaggagc aactgaagct gagtggggaa cattctgagt cttccactcc acgacccaga
     3841 tcatctcctg aagagacaat tgagcctgaa agtcttcacc agctctttga gggtgaaagt
     3901 gagaccgagt ctttctatgg ctttgaagaa gctgacctag atctgatgga gatttagtaa
     3961 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     4021 gacgatgaca agttggtgtc tagaaggcg
//
