LOCUS       sequence-280508-        4148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1353
FEATURES             Location/Qualifiers
     source          1..4148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggacac cgacctgttc atggaatgtg aggaggagga gttggagcca
       61 tggcagaaaa tcagtgatgt cattgaggac tctgtagttg aagattataa ttcagtggat
      121 aaaactacca cagctggcaa tcctttggtc cagcaaggtg gacagccact catcctgacc
      181 cagaatccag ccccaggtct gggcacaatg gttactcaac cagtattgag gcctgttcag
      241 gtcatgcaga atgccaatca tgtgactagt tcccctgtgg cctcacaacc aatatttatc
      301 actacgcagg gatttcctgt aaggaatgtc cggcctgtac aaaatgcaat gaatcaggtt
      361 gggattgtgc tgaacgtaca gcaaggccaa acggttagac caattacact agttccagcc
      421 ccaggtaccc agtttgttaa gccgacagtt ggagttccac aagtgttctc ccagatgacc
      481 cctgtgaggc caggctccac aatgcctgtg aggcccacca ccaacacctt caccaccgtc
      541 atcccggcca ctcttaccat tcgaagcacc gtcccacagt cccagtccca gcagaccaag
      601 tccactccca gcacttctac cactcccact gccacacagc caacctcact ggggcaacta
      661 gctgttcagt ctccaggcca gtcaaaccag accacgaatc ccaagctagt gagcattgcc
      721 agctttgtca ctgtgaagcg acctggtgtt acaggcgaaa atagcaatga agtggccaaa
      781 ttggtgaata cccttaacac catcccttcc ctgggccaga gtcctgggcc agtggtggtg
      841 tccaacaaca gctctgctca tggctctcaa agaaccagcg gacctgagtc ttcaatgaaa
      901 gtgacctctt ccatcccagt atttgacctc caggatggtg gacggaaaat atgtccacga
      961 tgtaatgctc aatttcgtgt tactgaagct ttgagaggtc acatgtgtta ctgttgccca
     1021 gaaatggttg aataccagaa gaaaggaaag tccctggatt cagaacccag tgtcccatca
     1081 gcagcaaagc ccccatcccc tgagaaaaca gctcctgttg cttccacacc ctcttctaca
     1141 cctattcctg ctctgtcacc gcctaccaaa gtaccagaac caaatgagaa cgtgggcgat
     1201 gccgtccaga ccaaactcat tatgcttgta gatgacttct actatggacg ggatggtggc
     1261 aaagtagccc agctcacaaa tttccctaag gtcgccacat ctttccgatg cccacattgt
     1321 accaaaaggc taaaaaacaa tattcgattc atgaaccata tgaaacacca cgtagaactc
     1381 gatcagcaga acggtgaggt agatggtcac actatctgcc agcactgtta ccgccagttt
     1441 tccactccct tccagcttca gtgccacttg gaaaatgttc atagtcccta tgaatctact
     1501 accaagtgca agatctgtga atgggcgttt gaaagtgagc cactatttct ccagcatatg
     1561 aaggatactc ataagcctgg agagatgcct tatgtttgcc aggtgtgtca atatcgctcc
     1621 tcactctact ctgaggtaga tgtccatttt cggatgatcc atgaggatac ccggcatctg
     1681 ctctgccctt attgcctgaa ggtcttcaaa aatggcaatg cattccaaca gcattacatg
     1741 aggcaccaga agagaaatgt ttatcactgc aacaaatgcc ggctgcagtt tctctttgcc
     1801 aaggacaaaa ttgaacacaa gcttcaacac cataaaacct tccgtaaacc caagcagctg
     1861 gagggcttga aaccaggcac caaggtgaca atccgggctt cccgagggca gccacgaact
     1921 gttcctgtat cctctaatga tacacctccc agcgccttgc aggaggcagc accgctgacc
     1981 tcctcaatgg accctctgcc tgtcttcctt tatccccctg tccagcgcag catccagaag
     2041 agagctgtta ggaaaatgag tgtcatgggc cggcagacat gcctggagtg cagcttcgag
     2101 atcccagact tccctaatca tttccctact tacgtacact gctctctgtg tcgctatagc
     2161 acctgctgtt ctcgagctta tgccaaccac atgatcaaca atcatgttcc acggaagagc
     2221 cccaagtatt tggctttgtt taaaaattct gtgagtggaa tcaagctggc ctgcacttca
     2281 tgtacctttg ttacctctgt gggcgatgct atggccaagc atttggtatt caacccctct
     2341 cacagatcca gcagcatcct gccacgggga ctcacttgga tagctcactc aaggcatggc
     2401 cagactcgtg accgagtgca tgaccggaac gtgaagaata tgtaccctcc tccttccttc
     2461 cccactaaca aagctgccac tgtgaaatct gcgggggcca ccccagctga gcctgaagag
     2521 ctactaactc ccttagcccc agcactccca tcaccagcct caactgcaac cccaccacca
     2581 acccccactc acccgcaggc tttagccctt ccaccgctgg ctacagaggg agccgaatgt
     2641 ctgaatgttg atgatcagga tgaagggagc ccagtcaccc aagaacctga gctagcatca
     2701 ggtggtggtg gtagtggtgg agttggcaaa aaggagcagc tgtctgtgaa gaagcttcga
     2761 gtagtactgt ttgctctatg ctgcaataca gaacaggcag ctgaacactt ccgaaatccc
     2821 cagcgacgta ttcgccgttg gcttcgacgt ttccaggcct cccaggggga gaatctagag
     2881 ggcaaatatc tgagctttga ggcagaagag aaactggctg agtgggtgct aacccagcgc
     2941 gaacaacagc tacctgtaaa tgaggagacc ttgttccaga aggccaccaa aataggacgt
     3001 tctttggaag gggggtttaa gatctcctat gagtgggctg tgcgtttcat gctgcggcac
     3061 cacctgactc cccatgcccg gcgagctgtg gcccacaccc tacctaagga tgtagcagag
     3121 aatgcaggac tcttcattga ttttgtacaa cggcagattc acaaccagga cttacccttg
     3181 tctatgattg tggctattga tgagatctct ttgttcctgg atacagaggt gctgagcagt
     3241 gatgatcgaa aggagaatgc cctgcagaca gtgggcacag gggaaccttg gtgtgatgta
     3301 gtcctagcca ttctggcaga tggcactgtc cttcccaccc tggttttcta cagagggcag
     3361 atggatcagc ctgctaacat gccagactcc atattgctag aggcaaagga gagtggctac
     3421 agtgatgacg agatcatgga gctgtggtca actcgagtgt ggcagaagca cacagcttgc
     3481 cagcgcagca aaggcatgct tgtgatggac tgtcatcgca ctcacttgtc agaagaggta
     3541 ctggctatgc ttagtgcctc tagcactttg cctgcagtgg tcccagcagg ctgtagctcc
     3601 aaaattcagc cattagatgt atgcatcaaa agaactgtca agaacttcct gcataaaaaa
     3661 tggaaggaac aggctcggga aatggcagat actgcatgtg attctgatgt cctgcttcag
     3721 ctggtgcttg tctggctggg tgaagtgcta ggtgtcattg gggactgtcc agagctagtt
     3781 cagcgctcct tcctggtggc tagtgttctg cctggccccg atggcaacat taactcacct
     3841 acaagaaatg ctgacatgca ggaggagcta attgcctccc tagaggagca actgaagctg
     3901 agtggggaac attctgagtc ttccactcca cgacccagat catctcctga agagacaatt
     3961 gagcctgaaa gtcttcacca gctctttgag ggtgaaagtg agaccgagtc tttctatggc
     4021 tttgaagaag ctgacctaga tctgatggag atttagtaat gaactagtcc gtaacttgaa
     4081 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagttgtt atcgaccttg
     4141 gcacattc
//
