LOCUS       sequence-280514-        1526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1370
FEATURES             Location/Qualifiers
     source          1..1526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaactctt aacattcagg gatgtggcca tagaattctc tccagaagag
       61 tggaaatgcc tggaccctga ccagcagaat ttgtatagag atgtgatgtt ggagaactac
      121 aggaacctgg tctccctggg tgttgctatc tctaacccag acctggtcac ctgtctggag
      181 caaagaaaag agccctacaa tgtgaagata cataagatcg tagccagacc cccagctatg
      241 tgttctcatt tcacccaaga ccattggcca gtgcagggca tagaagattc attccacaaa
      301 cttatactga gaagatatga gaaatgtgga catgataatt tacaattaag aaaaggctgt
      361 aaaagtttga atgagtgtaa gttgcagaaa ggaggttata atgaatttaa tgaatgcttg
      421 tcaactaccc agagcaaaat acttcagtgt aaagcaagtg tcaaagttgt tagtaaattt
      481 tcaaattcaa acaaacgtaa gacaagacat actggagaga aacactttaa agaatgtggc
      541 aaatcatttc agaagttttc acacctaact caacataagg taattcatgc tggagagaaa
      601 ccctacactt gtgaagaatg tggcaaagcc tttaaatggt ctttaatatt taatgaacat
      661 aagagaattc atactggaga gaaacctttt acttgtgaag aatgtggcag catctttacc
      721 acatcctcac actttgctaa gcataaaata attcatactg gagaaaaacc ctataaatgt
      781 gaagaatgtg gcaaagcctt taataggttc acaaccctta ctaaacataa gagaattcat
      841 gctggagaga aacccatcac atgtgaagaa tgtaggaaaa tctttacctc atcctcaaac
      901 tttgccaaac ataagcgaat tcatactgga gagaaaccct acaaatgtga agaatgtggc
      961 aaagccttta ataggtccac aacccttact aaacataaga gaattcatac tggagagaaa
     1021 ccctacacat gtgaagaatg tggcaaagct tttagacagt cctcaaaact gaatgaacat
     1081 aagaaagttc atactggaga gcggccctac aaatgtgatg aatgtggcaa agcctttgga
     1141 cggtccaggg tcctgaatga acataaaaaa attcatactg gagagaaacc ctacaaatgt
     1201 gaagaatgtg gcaaagcctt tagacggtcc acagatcgga gtcaacataa gaaaattcat
     1261 agtgcagata aaccctacaa atgtaaagaa tgtgacaaag cctttaaaca attttcgctc
     1321 ctgagtcaac ataagaaaat tcatactgta gataaaccct acaaatgtaa agattgtgac
     1381 aaagccttta aacggttctc acacctgaat aaacataaga aaattcatac ttagtaatga
     1441 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1501 gacggtattg ccattaccga tcctat
//
