LOCUS       sequence-280535-        2639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1465
FEATURES             Location/Qualifiers
     source          1..2639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagctgtg gcgccaatgc acccactggc tcatccagtg ccgggtgctg
       61 ccgcccagcc accgcgtgac ctgggatggg gctcaggtgt gtgaactggc ccaggccctc
      121 cgggatggtg tccttctgtg tcagctgctt aacaacctgc taccccatgc catcaacctg
      181 cgtgaggtca acctgcgccc ccagatgtcc cagttcctgt gccttaagaa cattagaacc
      241 ttcctgtcca cctgctgtga gaagttcggc ctcaagcgga gcgagctctt cgaagccttt
      301 gacctcttcg atgtgcagga ttttggcaag gtcatctaca ccctgtctgc tctgtcctgg
      361 accccgatcg cccagaacag ggggatcatg cccttcccca ccgaggagga gagtgtaggt
      421 gatgaagaca tctacagtgg cctgtccgac cagatcgacg acacggtgga ggaggatgag
      481 gacctgtatg actgcgtgga gaatgaggag gcggaaggcg acgagatcta tgaggacctc
      541 atgcgctcgg agcccgtgtc catgccgccc aagatgacag agtatgacaa gcgctgctgc
      601 tgcctgcggg agatccagca gacggaggag aagtacactg acacgctggg ctccatccag
      661 cagcatttct tgaagcccct gcaacggttc ctgaaacctc aagacattga gatcatcttt
      721 atcaacattg aggacctgct tcgtgttcat actcacttcc taaaggagat gaaggaagcc
      781 ctgggcaccc ctggcgcagc caatctctac caggtcttca tcaaatacaa ggagaggttc
      841 ctcgtctatg gccgctactg cagccaggtg gagtcagcca gcaaacacct ggaccgtgtg
      901 gccgcagccc gggaggacgt gcagatgaag ctggaggaat gttctcagag agccaacaac
      961 gggaggttca ccctgcggga cctgctgatg gtgcctatgc agcgagttct caaatatcac
     1021 ctccttctcc aggagctggt gaaacacacg caggaggcga tggagaagga gaacctgcgg
     1081 ctggccctgg atgccatgag ggacctggct cagtgcgtga acgaggtcaa gcgagacaac
     1141 gagacactgc gacagatcac caatttccag ctgtccattg agaacctgga ccagtctctg
     1201 gctcactatg gccggcccaa gatcgacggg gaactcaaga tcacctcggt ggaacggcgc
     1261 tccaagatgg acaggtatgc cttcctgctc gacaaagctc tactcatctg taagcgcagg
     1321 ggagactcct atgacctcaa ggactttgta aacctgcaca gcttccaggt tcgggatgac
     1381 tcttcaggag accgagacaa caagaagtgg agccacatgt tcctcctgat cgaggaccaa
     1441 ggtgcccagg gctatgagct gttcttcaag acaagagaat tgaagaagaa gtggatggag
     1501 cagtttgaga tggccatctc caacatctat ccggagaatg ccaccgccaa cgggcatgac
     1561 ttccagatgt tctcctttga ggagaccaca tcctgcaagg cctgtcagat gctgcttaga
     1621 ggtaccttct atcagggcta ccgctgccat cggtgccggg catctgcaca caaggagtgt
     1681 ctggggaggg tccctccatg tggccgacat gggcaagatt tcccaggaac tatgaagaag
     1741 gacaaactac atcgcagggc tcaggacaaa aagaggaatg agctgggtct gcccaagatg
     1801 gaggtgtttc aggaatacta cgggcttcct ccaccccctg gagccattgg accctttcta
     1861 cggctcaacc ctggagacat tgtggagctc acgaaggctg aggctgaaca gaactggtgg
     1921 gagggcagaa atacatctac taatgaaatt ggctggtttc cttgtaacag ggtgaagccc
     1981 tatgtccatg gccctcctca ggacctgtct gttcatctct ggtacgcagg ccccatggag
     2041 cgggcagggg cagagagcat cctggccaac cgctcggacg ggactttctt ggtgcggcag
     2101 agggtgaagg atgcagcaga atttgccatc agcattaaat ataacgtcga ggtcaagcac
     2161 attaaaatca tgacagcaga aggactgtac cggatcacag agaaaaaggc tttccggggg
     2221 cttacggagc tggtggagtt ttaccagcag aactctctaa aggattgctt caagtctctg
     2281 gacaccacct tgcagttccc cttcaaggag cctgaaaaga gaaccatcag caggccagca
     2341 gtgggaagca caaagtattt tggcacagcc aaagcccgct atgacttctg cgcccgagac
     2401 cgatcagagc tgtcgctcaa ggagggtgac atcatcaaga tccttaacaa gaagggacag
     2461 caaggctggt ggcgagggga gatctatggc cgggttggct ggttccctgc caactacgtg
     2521 gaggaagatt attctgaata ctgctagtaa tgaactagtc cgtaacttga aagtatttcg
     2581 atttcttggc tttatatatc ttgtggaaag gacgatgaaa ttcatgcgga acgtggtgc
//
