LOCUS       sequence-280538-        1832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1469
FEATURES             Location/Qualifiers
     source          1..1832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgtgag gttctacccc gcggcggccg gggaccctgc cagcctggac
       61 ttcgcgcagt gcctggggta ctacggctac agcaagtttg gaaataataa taactatatg
      121 aatatggctg aggcgaacaa tgcgttcttc gctgccagtg agcagacatt ccacacacca
      181 agccttgggg acgaggaatt cgaaattcca ccaatcacgc ctcctccaga gtcagaccct
      241 gccctaggca tgccggatgt actgctaccc tttcaagccc tcagcgatcc attgccttcc
      301 cagggaagtg aattcacacc ccagtttccc cctcaaagcc tggacctccc ttccattaca
      361 atctcaagaa atctcgtgga acaagatggc gtgcttcata gcagtgggtt gcatatggat
      421 cagagccaca cacaagtgtc ccagtaccgg caggatccct ccctgatcat gcggtccatc
      481 gtccacatga ccgatgctgc gcgttctggg gtcatgcctc ctgcccagct caccaccatc
      541 aaccagtctc agctcagcgc ccagttgggg ttgaatttgg gaggtgccag tatgcctcac
      601 acatctcctt cacctccagc aagcaaatca gccactccct ccccttccag ctccatcaat
      661 gaagaggatg ctgatgaagc caacagagcc attggagaga aaagagctgc tccagactct
      721 ggcaagaagc ccaagactcc aaagaaaaag aaaaagaaag atcccaatga gccacagaag
      781 ccagtgtcag catatgccct gtttttcaga gacacacagg ctgcaattaa aggtcaaaac
      841 cccaatgcaa cctttggaga ggtctcaaaa attgtagcat ctatgtggga cagccttgga
      901 gaagaacaaa agcaggtata taaaaggaaa acagaagctg ccaaaaaaga atacctgaag
      961 gccctggcgg catacagggc cagcctcgtt tctaaggctg ctgctgagtc agcagaagcc
     1021 cagaccatcc gttctgttca gcagaccctg gcgtcgacca atctaacatc ctctctcctt
     1081 ctcaacactc cactgtctca acatggaaca gtgtcagcat cacctcagac tctccagcaa
     1141 tccctcccta ggtcaatcgc tcccaaaccc ttaaccatga gactccccat gaaccagatt
     1201 gtcacatcag tcaccattgc agccaacatg ccctcgaaca ttggggctcc actgataagc
     1261 tccatgggaa cgaccatggt tggctcagca ccctccaccc aagtgagtcc ttcggtgcaa
     1321 acccagcagc atcagatgca attgcagcag cagcagcagc agcaacaaca acagatgcaa
     1381 cagatgcagc agcagcaact ccagcagcac caaatgcatc agcaaatcca gcagcagatg
     1441 cagcagcagc atttccagca ccacatgcag cagcacctgc agcagcagca gcagcatctc
     1501 cagcagcaaa ttaatcaaca gcagctgcag cagcagctgc agcagcgcct ccagctgcag
     1561 cagctgcaac acatgcagca ccagtctcag ccttctcctc ggcagcactc ccctgtcgcc
     1621 tctcagataa catcccccat ccctgccatc gggagccccc agccagcctc tcagcagcac
     1681 cagtcgcaaa tacagtctca gacacagact caagtattat cgcaggtcag tattttctag
     1741 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1801 aaggacgagg tcaacctata ttgcgattac tg
//
