LOCUS       sequence-28056-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Juven-Gershon T, Cheng S, Kadonaga JT
  TITLE     Rational design of a super core promoter that enhances gene 
            expression.
  JOURNAL   Nat Methods. 2006 Nov . 3(11):917-22.
  PUBMED    17124735
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2006 Nov . 3(11):917-22."
COMMENT     Sequence Label: SCP2-pUC119
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(94..118)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(168..185)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(168..184)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(177..199)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      397..853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(484..503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     694..715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttcacacag gaacagctat gaccatgatt acgccaagct tgcatgcctg cagttctatg
       61 gaggcacaac cactcggctc gacgtctcca ggcgatctga cggttcacta aacgagctct
      121 gcttatatag accttctaga ggatccccgg gtaccgagct cgaattcact ggccgtcgtt
      181 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      241 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      301 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
      361 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
      421 gcgcggcggg tgtggtggtt ncgcgcagcg tgaccgctac acttgccagc gccttagcgc
      481 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      541 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      601 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      661 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      721 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
      781 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaaatatta
      841 acgtttacaa ttttatggtg c
//
