LOCUS       sequence-280568-        1433 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1613
FEATURES             Location/Qualifiers
     source          1..1433
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaccgttt gggttccttt agcaatgatc cctctgataa gccaccttgc
       61 cgaggctgct cctcctacct catggagcct tatatcaagt gtgctgaatg tgggccacct
      121 ccttttttcc tctgcttgca gtgtttcact cgaggctttg agtacaagaa acatcaaagc
      181 gatcatactt atgaaataat gacttcagat tttcctgtcc ttgatcccag ctggactgct
      241 caagaagaaa tggccctttt agaagctgtg atggactgtg gctttggaaa ttggcaggat
      301 gtagccaatc aaatgtgcac caagaccaag gaggagtgtg agaagcacta tatgaagcat
      361 ttcatcaata accctctgtt tgcatctacc ctgctgaacc tgaaacaagc agaggaagca
      421 aaaactgctg acacagccat tccatttcac tctacagatg accctccccg acctaccttt
      481 gactccttgc tttctcggga catggccggg tacatgccag ctcgagcaga tttcattgag
      541 gaatttgaca attatgcaga atgggacttg agagacattg attttgttga agatgactcg
      601 gacattttac atgctctgaa gatggctgtg gtagatatct atcattccag gttaaaggag
      661 agacaaagac gaaaaaaaat tataagagac catggattaa tcaaccttag aaagtttcaa
      721 ttaatggaac ggcggtatcc caaggaggtc caggacctgt atgaaacaat gaggcgattt
      781 gcaagaattg tggggccagt ggaacatgac aaattcattg aaagccatgc attggaattt
      841 gaactccgaa gggaaatcaa gaggctccaa gaatacagga cagcaggcat taccaatttt
      901 tgtagtgcca gaacctacga tcacctcaag aagacacggg aggaagagcg ccttaaacgc
      961 actatgctct cagaagttct ccagtatatc caggacagta gtgcttgcca gcagtggctc
     1021 cgccggcaag ctgacattga ttccggcctg agtccttcca ttccaatggc ttcgaattca
     1081 ggtagacgga gtgcaccacc cttgaacctc actggcctcc ctggcacaga gaagctgaat
     1141 gaaaaagaaa aggagctctg tcagatggtg aggttggtcc ctggagccta tttagaatac
     1201 aaatctgctc tattgaacga atgtaacaag caaggaggct taagactggc gcaggcaaga
     1261 gcactcatca agatagatgt gaacaaaacc cggaaaatct atgatttcct catcagagaa
     1321 ggatacatca ctaaaggcta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1381 tggctttata tatcttgtgg aaaggacgaa ttacaagccg taggatgcct gct
//
