LOCUS       sequence-28057-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Juven-Gershon T, Cheng S, Kadonaga JT
  TITLE     Rational design of a super core promoter that enhances gene 
            expression.
  JOURNAL   Nat Methods. 2006 Nov . 3(11):917-22.
  PUBMED    17124735
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2006 Nov . 3(11):917-22."
COMMENT     Sequence Label: SCP1-CMVenh-luc
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(76..95)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(77..95)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(186..206)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        361..664
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca agcttcccta tagtgagtcg tattacggtc cgtaggcacg tctgctcggc
      121 tcgagtgttc gatcgcgact gaggacgaac gcgcccccac ccccttatat aagtacccca
      181 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
      241 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
      301 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
      361 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
      421 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
      481 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
      541 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
      601 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
      661 aacgcggaac tccatatatg ggctatgaac taatgacccc c
//
