LOCUS       sequence-28058-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Juven-Gershon T, Cheng S, Kadonaga JT
  TITLE     Rational design of a super core promoter that enhances gene 
            expression.
  JOURNAL   Nat Methods. 2006 Nov . 3(11):917-22.
  PUBMED    17124735
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2006 Nov . 3(11):917-22."
COMMENT     Sequence Label: SCP1-CMVenh-luc
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(185..203)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gggggcgcgt tcgtcctcag tcgcgatcga acactcgagc cgagcagacg tgcctacgga
       61 ccgtaatacg actcactata gggaagcttg gcattccggt actgttggta aagccaccat
      121 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
      181 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
      241 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
      301 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
      361 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
      421 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
      481 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
      541 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
      601 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
      661 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
      721 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
      781 tgccagag
//
