LOCUS       sequence-280580-        2360 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1644
FEATURES             Location/Qualifiers
     source          1..2360
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgagcc ggcttcggta actgtgacat ttgatgatgt ggccttatat
       61 ttttcggaac aagagtggga gatcctggag aagtggcaga agcaaatgta taagcaagag
      121 atgaagacca attacgagac ccttgattcc ctggggtatg ctttttccaa gccagatttg
      181 atcacatgga tggaacaagg gagaatgcta ttaatcagcg aacagggatg cttagataaa
      241 acaagaagga caactagccc tcctactgat gaacagttga acatgaagaa tactggaaag
      301 ttgctatgtt ttgatgacga aggaactccc aggacaaaag aagaggattg ccgtttaaat
      361 ggtcctcaaa aacaggactt gtgtgctgcc ttacgaggga aagagagaaa gattttatta
      421 gctcaaacag ccaccttcca gtctccaagt ctccgagaaa cagagattct aaataaaaaa
      481 gtcagcatca cagcatatga tccagacaag aaagacctgc ggcataagcc tcgggagacc
      541 ccagggcgct tagaaattcc cacagggcca agatgctatt cctgctatgt ctgtaggaaa
      601 gtcttccaag taaggaggga tttgctaaag cacaaacgga gccactccaa gagccagctc
      661 tgcagatacc caaagtacaa aaactcgtcc agagggaagt ccgaactcag gcggacgcag
      721 aggctcctgt gtcagaagaa gcggttccag tgcagtgagt gtgagaagag ctacttcctg
      781 aagggcagcc tcgtcactca tcaggttgtc cacaccggcc agcggcccta cccatgccct
      841 gagtgcgaca agaccttccg gtacagggcc aacctgaaga agcacctgtg tctacaccgc
      901 ggggagcggc cgttctgctg cggggagtgc ggccgggcct tcgtgcagca gtgcgagctc
      961 acggagcact tgcggctgca cagcggagag aagcccttcc agtgtccgca gtgtgaccgg
     1021 tgcttccgcc tgaagagggg catgaaggtc catctgaccc agcacagcgg gaagaggccc
     1081 ttccactgtc ccgagtgtgg ccggagcttc tcccggaagg ctgccctgaa gacccaccag
     1141 aggacgcaca gcgaggaaaa gccgttttct tgtggtgaat gtggcaggaa attcatctac
     1201 aagattaagc tggacgagca catcagagtt cacacgggag agaagccctt ttcgtgtccc
     1261 gagtgtaaca aaagtttccg cctcaagaga agcctgaaag cccacgggct gcagcacatt
     1321 gggaagcggc ccttccagtg cccggagtgc agcaggggct tcttctggag gaacgccatg
     1381 cgcgcccacc agcgcctgca cagcgagcaa aagcccttcc cctgcgccga gtgcggcaag
     1441 cgcttcacgc ggccttccaa gctcgcctgc cacaccagag tccacgacag gcagaaggag
     1501 tttccctgcg gcgagtgcaa aaagaccttt tctcagcagt cgcggctcac gcagcacctg
     1561 aaggtccaca ccacggaaaa gccgttctcc tgcgccgagt gcggccgcag tttccgccga
     1621 cgcgcgcatc tcacagagca cacgaggctt cacagtggcg aggagccctt ccagtgtccc
     1681 gagtgcgaca agagcttctc ctggaaggcc tccatgaagt tccaccagcg gatgcacagg
     1741 gacgagaagc ccttcgcgtg cggtgagtgt gacaagacct acacgcatca gtctcagctc
     1801 accgagcacc tgaggctgca cagtggagag aagccctacc agtgtcctga atgcgagaag
     1861 actttccgcc tcaagggaaa cctgaaaagc cacctgctgc agcacagtgg ccaaaagcca
     1921 ttctcttgtg tgatgtgcgg caaaagtttc actcaacagt accggctcac agaacacatt
     1981 cgagtccaca gcggggagaa gcccttccag tgtccggagt gtgacaagag ctactgcatc
     2041 aggggcagct tgaaggtcca cttgtataag cacagtggag agaggccctt ccagtgtccc
     2101 gagtgtggca aaggcttcct ccagaagaga agcctgaagg cccatctgtg ccttcacagt
     2161 ggggagaggc ctttttcttg tgatgagtgt ggaaggagct tcacgtacgt gggggcgctc
     2221 aagacccaca ttgcagtgca tgccaaagag aagccctcca gcctctagta atgaactagt
     2281 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatgga
     2341 tcatttcgca actatgttaa
//
