LOCUS       sequence-280586-        1589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1650
FEATURES             Location/Qualifiers
     source          1..1589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcaaa ttgcacatcc tcatggagtc taggagaatc ctgcaacaga
       61 cctgggttgg agctgccacg gtctatggca tcctcagaaa ctcaacttgg aaatcacgac
      121 gtggaccctg agatttctca cgtgaacttc aggatgttca gctgcccgaa ggagtcggac
      181 cccatccagg ctctgaggaa actcactgag ctgtgccatc tgtggctgag gcccgacctc
      241 cacaccaaag agcagatcct ggacatgctg gtgatggagc agttcatgat ctccatgccc
      301 caggagctcc aggtcttagt catgatgaac ggtgtgcaga gctgcaaaga cctggaggac
      361 ctgctacgaa ataacagaag acccaagaaa tggtctgtgg tcaccttcca cggaaaggaa
      421 tatattgtgc aggactcaga tatcgagatg gctgaagccc cctccagtgt cagagatgat
      481 ctgaaagacg tgtccagcca acgggcctcc tcggtgaacc agatgcgtcc aggggaaggc
      541 caggcccacc gagagctgca gatcctgccc agggtccctg cattgtccag gaggcaggga
      601 gaggactttc tgctacacaa gagtattgac gtaacaggtg acccaaagtc tctgagaccc
      661 aagcagacct tggagaagga tctgaaggaa aacagggaag agaacccagg actgacatcc
      721 ccagagcctc agcttccaaa gagtcccaat ctggtgagag caaaggaggg gaaggacccc
      781 ccaaaaatag cctctgtgga aaatgtggat gctgacacac cttctgcctg cgttgtggag
      841 agagaagctt cgactcacag cgggaacaga ggagacgctc tgaatctgag cagtcccaaa
      901 agaagcaaac cagatgcctc ctccatttcc caagaagagc ctcaaggaga agccacacct
      961 gtgggcaaca gagaatcccc gggacaagct gggatgaatt caattcattc cccaggccct
     1021 gcgagcccag tcagtcaccc ggatggccaa gaagccaagg cactgccgcc ctttgcatgt
     1081 gacgtgtgcg agaagaggtt tacgtgtaat tccaagctag tcatccacaa gagatcacac
     1141 acaggcgaga gactctttca atgtaatctc tgtgggaagc gcttcatgca gcttattagc
     1201 ctccaatttc accagcgaac ccacactggc gagaggccct acacgtgtga cgtctgccag
     1261 aagcagttca cccagaagtc ctacttgaag tgtcacaaga gaagccacac aggggagaag
     1321 cccttcgaat gtaaagactg caagaaagtt ttcacctaca gggggagcct gaaggagcac
     1381 cagcgcatcc actccggaga gaaaccctac aaatgttcca agtgtccaag agccttcagt
     1441 cggctgaaat tgttaagacg ccaccagaaa acacatccag aagctacttc tcagtagtaa
     1501 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1561 gacgaatagt tagggcgact gctcgggta
//
