LOCUS       sequence-280592-         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1657
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtcttc tatgacctgg acagctgcaa gcattccagc taccctgatt
       61 cagagggggc tcctgaccct ggttcccccg gcatatgccc cgtaccccag ccctgtgcta
      121 tcagaggagg aagacttacc gttggacagc cctgccctgg aggtctcgga cagcgagtcg
      181 gatgaggccc tcgtggctgg ccccgagggg aagggatccg aggcagggac tcgcaagaag
      241 ctgcgcctgt accagttcct gctggggcta ctgacgcgcg gggacatgcg tgagtgcgtg
      301 tggtgggtgg agccaggcgc cggcgtcttc cagttctcct ccaagcacaa ggaactcctg
      361 gcgcgccgct ggggccagca gaaggggaac cgcaagcgca tgacctacca gaagctggcg
      421 cgcgccctcc gaaactacgc caagaccggc gagatccgca aggtcaagcg caagctcacc
      481 taccagttcg acagcgcgct gctgcctgca gtccgccggg cctagtaatg aactagtccg
      541 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagtacata
      601 tagccgcgga ggatgct
//
