LOCUS       sequence-280595-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1658
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagaggt ggccagacaa gcacaaacct ctcaggagcc ctaccacgac
       61 aaggccagag aacaccccga tgacggaaag catccagatg gaggcctcta caacaaggga
      121 ccctcctact cgagttattc cgggtacata atgatgccaa atatgaataa cgacccatac
      181 atgtcaaatg gatctctttc tccacccatc ccgagaacat caaataaagt gcccgtggtg
      241 cagccatccc atgcggtcca tcctctcacc cccctcatca cttacagtga cgagcacttt
      301 tctccaggat cacacccgtc acacatccca tcagatgtca actccaaaca aggcatgtcc
      361 agacatcctc cagctcctga tatccctact ttttatccct tgtctccggg tggtgttgga
      421 cagatcaccc cacctcttgg ctggttttcc catcatatga ttcccggtcc tcctggtccc
      481 cacacaactg gcatccctca tccagctatt gtaacacctc aggtcaaaca ggaacatccc
      541 cacactgaca gtgacctaat gcacgtgaag cctcagcatg aacagagaaa ggagcaggag
      601 ccaaaaagac ctcacattaa gaagcctctg aatgctttta tgttatacat gaaagaaatg
      661 agagcgaatg tcgttgctga gtgtactcta aaagaaagtg cagctatcaa ccagattctt
      721 ggcagaaggt ggcatgccct ctcccgtgaa gagcaggcta aatattatga attagcacgg
      781 aaagaaagac agctacatat gcagctttat ccaggctggt ctgcaagaga caattatggt
      841 aagaaaaaga agaggaagag agagaaacta caggaatctg catcaggtac aggtccaaga
      901 atgacagctg cctacatcta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
      961 tggctttata tatcttgtgg aaaggacgag ctctaaggtg agattcgctc tac
//
