LOCUS       sequence-280601-        1682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1663
FEATURES             Location/Qualifiers
     source          1..1682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      644..653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgaaggagcg acacacgccc atcgagaggg tgccccatct cggcttcaag
       61 cagattaacc tgtggaagat ctacaaagca gtggagaagc tgggggccta tgagctggtg
      121 accgggcgcc gcctctggaa gaacgtgtac gacgagctgg ggggcagccc aggcagcacc
      181 agcgcggcca cgtgcacgcg ccgccactac gagaggctgg tcctgccata cgtgcggcac
      241 ctgaaggggg aggatgacaa gccgctgccc acctccaagc ccaggaaaca gtacaagatg
      301 gctaaggaga acagggggga tgatggggcc accgagaggc cgaagaaggc caaggaggag
      361 cggcgcatgg accagatgat gccaggaaag accaaagcag atgctgctga cccagcacca
      421 cttcccagcc aggagccccc caggaacagc acagaacagc agggcctggc ctctgggtct
      481 tctgtgtcct ttgtgggtgc cagcggctgt cctgaggcct acaagcggct cctatccagc
      541 ttctactgca aggggacaca cggcatcatg tcaccactgg ccaaaaagaa gctcctggcc
      601 caggtgagca aggtggaggc cttgcagtgc caggaggagg gctgccgcca tggggcagag
      661 ccccaggcgt ccccagctgt tcacctccca gagagtcccc agagccccaa agggctgact
      721 gagaactcca ggcaccggct gacccctcag gagggattgc aggccccagg tggcagcctc
      781 agagaggagg cgcaggcagg cccctgcccg gcagccccca tcttcaaggg ctgcttctac
      841 acccacccca ccgaggtgct gaagcctgtc agccagcacc ccagggactt cttctctaga
      901 cttaaagatg gggtgctatt ggggcctcct ggcaaagagg ggctgtcagt gaaagagccc
      961 cagctggtgt ggggcggaga cgctaaccgc ccttctgcgt tccataaagg tggctccaga
     1021 aagggcatcc tctaccccaa gcccaaagcc tgctgggtgt cccccatggc caaggtccca
     1081 gccgagagcc ccacgctccc gcccaccttc cccagtagcc caggcctggg cagcaagcgc
     1141 agcctggagg aagagggtgc tgcccacagt gggaagagac tgcgggccgt gtctcccttt
     1201 cttaaggagg cggatgccaa gaagtgtggg gccaaacctg cagggtccgg cctggtctcc
     1261 tgccttctgg gcccagccct ggggcctgtg cccccagagg cctacagggg caccatgctg
     1321 cactgcccgc tgaacttcac tggcaccccg ggccccttga agggccaggc tgcactcccc
     1381 ttcagccccc tggtcatccc ggccttcccg gcccacttcc tggccaccgc aggcccctcg
     1441 cccatggccg ctggcctgat gcacttcccc ccaacgtcct tcgacagtgc cctccgccac
     1501 agactttgcc cggcctcatc tgcctggcac gcaccaccag tcacaaccta tgcagcgccc
     1561 cacttcttcc acctcaacac caagctgtag taatgaacta gtccgtaact tgaaagtatt
     1621 tcgatttctt ggctttatat atcttgtgga aaggacgaaa cccgcttgcg atctcgatat
     1681 cc
//
