LOCUS       sequence-280619-        1121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1691
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagggca gcacaactac ttatgtgctg gaagaaatga ctgcatcgtt
       61 gataaaatcc gcagaaaaaa ctgcccagca tgtcgcctta gaaagtgctg tcaggctggc
      121 atggtccttg gaggtcgaaa atttaaaaag ttcaataaag tcagagttgt gagagcactg
      181 gatgctgttg ctctcccaca gccagtgggc gttccaaatg aaagccaagc cctaagccag
      241 agattcactt tttcaccagg tcaagacata cagttgattc caccactgat caacctgtta
      301 atgagcattg aaccagatgt gatctatgca ggacatgaca acacaaaacc tgacacctcc
      361 agttctttgc tgacaagtct taatcaacta ggcgagaggc aacttctttc agtagtcaag
      421 tggtctaaat cattgccagg ttttcgaaac ttacatattg atgaccagat aactctcatt
      481 cagtattctt ggatgagctt aatggtgttt ggtctaggat ggagatccta caaacacgtc
      541 agtgggcaga tgctgtattt tgcacctgat ctaatactaa atgaacagcg gatgaaagaa
      601 tcatcattct attcattatg ccttaccatg tggcagatcc cacaggagtt tgtcaagctt
      661 caagttagcc aagaagagtt cctctgtatg aaagtattgt tacttcttaa tacaattcct
      721 ttggaagggc tacgaagtca aacccagttt gaggagatga ggtcaagcta cattagagag
      781 ctcatcaagg caattggttt gaggcaaaaa ggagttgtgt cgagctcaca gcgtttctat
      841 caacttacaa aacttcttga taacttgcat gatcttgtca aacaacttca tctgtactgc
      901 ttgaatacat ttatccagtc ccgggcactg agtgttgaat ttccagaaat gatgtctgaa
      961 gttattgctg cacaattacc caagatattg gcagggatgg tgaaacccct tctctttcat
     1021 aaaaagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1081 tcttgtggaa aggacgagaa tcgatacgga cccagttgaa c
//
