LOCUS       sequence-280625-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1697
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatcttgt cagatctgct cccgggggta ttcttgactt aaacaaggtt
       61 gcaacgaaac tgggagtccg aaagcggaga gtgtatgaca tcaccaatgt cttagatgga
      121 atcgacctcg ttgaaaagaa atccaagaac catattagat ggataggatc tgatcttagc
      181 aattttggag cagttcccca acaaaagaag ctacaggagg aactttctga cttatcagca
      241 atggaagatg ctttggatga gttaattaag gattgtgctc agcagctgtt tgagttaaca
      301 gatgacaaag aaaatgaaag actagcatat gtgacctatc aagacattca tagcattcag
      361 gccttccatg aacagatcgt cattgcagtt aaagctccag cagaaaccag attggatgtt
      421 ccagctccca gagaagactc tatcacagtg cacataagga gcaccaacgg acctatcgat
      481 gtctatttgt gtgaagtgga gcagggtcag accagtaaca aaaggtctga aggtgtcggg
      541 acctcttcat ctgagagcac tcatccagaa ggccctgagg aagaagaaaa tcctcagcaa
      601 agtgaagaat tgcttgaagt aagcaactag taatgaacta gtccgtaact tgaaagtatt
      661 tcgatttctt ggctttatat atcttgtgga aaggacgagg tacttatcgt gatcggtaaa
      721 tg
//
