LOCUS       sequence-280649-        1526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1727
FEATURES             Location/Qualifiers
     source          1..1526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcttcctc cccggcggtg gacgtgtcct gcaggcggcg ggagaagcgg
       61 cggcagctgg acgcgcgccg cagcaagtgc cgcatccgcc tgggcggcca catggagcag
      121 tggtgcctcc tcaaggagcg gctgggcttc tccctgcact cgcagctcgc caagttcctg
      181 ttggaccggt acacttcttc aggctgtgtc ctctgtgcag gtcctgagcc tttgcctcca
      241 aaaggtctgc agtatctggt gctcttgtct catgcccaca gccgagagtg cagcctggtg
      301 cccgggcttc gggggcctgg cggccaagat ggggggcttg tgtgggagtg ctcagcaggc
      361 cataccttct cctggggacc ctctttgagc cctacacctt cagaggcacc caagccagcc
      421 tcccttccac atactactcg gagaagttgg tgttccgagg ccacgagtgg gcaggagctt
      481 gcagatttgg aatctgagca tgatgagagg actcaagagg ccaggttgcc cagtagtgag
      541 cctgatgccc ccagactact gccttcccct gtcacctgca cacctaaaga gggggagaca
      601 ccaccagccc ctgcagcact ctccagtcct cttgctgtgc cggccttgtc agcatcctca
      661 ttgagttcca gagctcctcc acctgcagaa gtcagggtgc agccacagct cagcaggacc
      721 cctcaagcgg cccagcagac tgaggccctg gccagcactg ggagtcaggc ccagtctgct
      781 ccaaccccgg cctgggatga ggacactgca caaattggcc ccaagagaat taggaaagct
      841 gccaaaagag agctgatgcc ttgtgacttc cctggctgtg gaaggatctt ctccaaccgg
      901 cagtatttga atcaccacaa aaagtaccag cacatccacc agaagtcttt ctcctgccca
      961 gagccagcct gtgggaagtc tttcaacttt aagaaacacc tgaaggagca catgaagctg
     1021 cacagtgaca cccgggacta catctgtgag ttctgcgccc ggtctttccg cactagcagc
     1081 aaccttgtca tccacagacg tatccacact ggagaaaaac ccctgcagtg tgagatatgc
     1141 gggtttacct gccgccagaa ggcttccctg aactggcacc agcgcaagca tgcagagacg
     1201 gtggctgcct tgcgcttccc ctgtgaattc tgcggcaagc gctttgagaa gccagacagt
     1261 gttgcagccc accgtagcaa aagtcaccca gccctgcttc tagcccctca agagtcaccc
     1321 agtggtcccc tagagccctg tcccagcatc tctgcccctg ggcctctggg atccagcgag
     1381 gggtccaggc cctctgcatc tcctcaggct ccaaccctgc ttcctcagca atagtaatga
     1441 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1501 gagaagtcac aactaaccaa gtgtgc
//
