LOCUS       sequence-280655-        1721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1737
FEATURES             Location/Qualifiers
     source          1..1721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(524..535)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggggagccc cgaggatgac ctgattggga ttccattccc ggaccacagc
       61 agtgagctcc tgagctgcct caatgagcag cgccagctgg gccacctatg tgacctcacc
      121 atccggacgc agggccttga ataccgcacc cacagggctg tgctagctgc ctgtagccac
      181 tacttcaaga agcttttcac tgagggcggt ggcggagctg tcatgggggc cgggggtagc
      241 gggacggcca ctgggggagc aggggccggt gtgtgtgagc tggactttgt agggccagag
      301 gcactaggcg ccctccttga atttgcctat acagccacac tgaccaccag cagcgccaac
      361 atgccagctg tgctccaggc tgcccgcctg ctggagatcc cgtgtgtcat cgctgcttgc
      421 atggagattc tgcagggcag tgggctagaa gctcccagcc cggacgagga tgactgtgag
      481 cgagcccgcc agtatctgga ggcctttgcc acagccacgg cctctggagt tcccaatggt
      541 gaagacagtc ctccacaggt gcccctccca ccacctccgc caccgccacc tcggcctgtt
      601 gcccgccgca gccgcaagcc ccggaaagct ttcctgcaaa ccaagggggc cagagcaaac
      661 cacctagtcc ctgaggtgcc cacagtgccc gcccatccct tgacctatga ggaggaggag
      721 gtggcgggca gagtgggcag cagtgggggc agtgggccgg gggacagcta cagccctccc
      781 acaggaactg cctcccctcc tgagggtccc cagagctacg aaccctatga gggtgaggaa
      841 gaagaagagg agctggtata tcccccagcc tatgggctgg cgcagggtgg cgggcccccg
      901 ctgtccccag aggagctggg ctcagatgag gatgccatcg atcctgacct gatggcctac
      961 ctaagctccc tgcaccagga caacctggca ccaggcctgg acagccaaga caagctggtg
     1021 cgcaaacgcc gctcccagat gcctcaggag tgccctgtct gccacaagat catccatggg
     1081 gcaggcaaac tgcctcgcca catgaggacc cacacaggcg agaagccctt tgcctgcgag
     1141 gtctgcggtg ttcgattcac caggaacgac aagctgaaga tccacatgcg gaagcacacg
     1201 ggagagcgcc cctactcatg cccgcactgc ccagcccgct tcctgcacag ctacgacctc
     1261 aagaaccaca tgcacctgca cacaggggac cggccctatg agtgccacct gtgccacaag
     1321 gctttcgcca aggaggacca cctgcagcgc cacctcaaag gccagaactg cctggaggtg
     1381 cgcacccgac ggcgccgcaa ggacgatgca ccaccccact acccaccacc ctctaccgct
     1441 gctgcatccc ccgctggcct cgacctctcc aatggccacc tggacacctt ccgcctctct
     1501 ctagctcgat tctgggagca gtcagccccc actgggcccc cggtctctac cccagggccc
     1561 cctgatgacg atgaggagga aggggcaccc accacacccc aggctgaagg tgccatggag
     1621 tcctcttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1681 tcttgtggaa aggacgaagg cggtgacgat catacgcccg a
//
