LOCUS       sequence-280658-        1559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1739
FEATURES             Location/Qualifiers
     source          1..1559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctacaga accaaagaaa gctgcagccc agaactctcc agaggatgaa
       61 ggacttctga tagtgaagat agaagaggaa gaatttatcc atgggcagga cacttgctta
      121 cagagaagtg aactccttaa gcaggagctc tgcaggcagc tttttaggca gttctgctac
      181 caggattctc ctggacctcg cgaggcactg agccgcctcc gggagctctg ctgtcagtgg
      241 ctgaagccag agatccatac caaggaacag attctggaac tgctggtgct agagcagttc
      301 ctgaccatcc tgccaggaga tttgcaggcc tgggtacatg aacattaccc agagagtgga
      361 gaggaggcag tgaccatact agaagatttg gagagaggca ctgatgaagc agtactccag
      421 gttcaagccc atgaacatgg acaagaaata ttccagaaaa aagtgtcacc tcctggacca
      481 gcacttaatg tcaagttaca gccagtggag accaaggccc attttgattc atcagaaccc
      541 cagctcctat gggactgtga taatgagagt gaaaacagta gatccatgcc aaagctggaa
      601 atttttgaaa aaattgaatc acagagaatt atatctggaa gaatctcagg atacatatca
      661 gaagcatctg gtgagtctca agacatctgt aagtctgcag gcagggtaaa gagacaatgg
      721 gaaaaagaat caggggagtc tcagagactc tcgtctgccc aggatgaagg ttttggtaaa
      781 atcctcaccc acaaaaatac agtcagaggt gaaataataa gccacgatgg atgtgagagg
      841 agattaaatc tgaactcaaa tgaattcaca caccagaaat cttgtaaaca tggtacctgt
      901 gaccagagct tcaaatggaa ctcagatttt attaaccatc aaataattta tgctggagaa
      961 aaaaatcacc aatatggaaa atctttcaag agcccaaaac ttgctaaaca tgcagcagtt
     1021 ttcagtggag ataaaactca tcagtgtaat gaatgtggga aagctttcag gcacagctca
     1081 aaacttgcta ggcatcagag aatccacact ggagagagat gctatgaatg taatgaatgt
     1141 gggaaaagct ttgcagagag ctcagatctt actagacatc ggcgaattca cactggggaa
     1201 agaccctttg gttgcaaaga atgtgggaga gcattcaacc tgaactcaca tcttatcagg
     1261 catcagagaa ttcacaccag agagaaaccc tacgagtgta gtgaatgtgg gaaaaccttc
     1321 cgagtgagct cacatcttat tcgacacttt agaattcaca ctggagaaaa accctatgaa
     1381 tgcagtgagt gtggaagagc cttcagtcag agctcaaacc ttagtcaaca ccagagaatt
     1441 cacatgaggg aaaacctatt aatgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1501 atttcttggc tttatatatc ttgtggaaag gacgaagttc agcaaggtgc taatactgg
//
