LOCUS       sequence-280661-        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1743
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgccgcc tctggccccg ctccctcccc gggacccaaa cggggccgga
       61 cccgagtgga gggagccggg ggctgtgagc ttcgcggacg tggccgtgta cttctgccgg
      121 gaggagtggg gctgcttgcg gccagcgcag agggccctgt accgggacgt gatgcgggag
      181 acctacggcc acctgagcgc tctcggaatc ggaggcaaca agccagctct catctcctgg
      241 gtggaggagg aggccgaact gtggggtccg gctgcccagg atccggaggt ggcgaaatgt
      301 cagacacaaa cggacccaga ttccagaaac aagaaaaagg aaagacaaag ggaagggacg
      361 ggagccctgg agaagcccga ccctgtggcc gccgggtctc ctgggctgaa gtctccccaa
      421 gccccctcgg ccgggccccc ttatggttgg gagcagctgt ccaaggcacc gcaccgggga
      481 cgcccctccc tctgtgccca cccccctgtc ccccgagctg accagcgtca tggctgctac
      541 gtgtgcggga agagctttgc ctggcgctcc acactggtgg agcacgtcta cagtcacact
      601 ggcgagaagc ccttccactg cactgactgc ggcaagggct tcggccacgc ttcctccctg
      661 agcaaacacc gggccatcca tcgtggggag cggccccacc gctgtctgga gtgtggccgg
      721 gccttcacgc agcgctcggc gctgacttcg cacctgcgcg tccacaccgg cgagaaaccc
      781 tatggctgcg ccgactgtgg ccgccgcttc agccagagct ctgccctcta ccagcaccgg
      841 cgcgtgcaca gcggcgagac ccccttcccc tgcccggact gtggccgcgc cttcgcctac
      901 ccctcggacc tgcggcgcca cgtgcgcacc cacaccggcg agaagcccta cccgtgcccg
      961 gactgcgggc gctgcttccg ccagagctcg gagatggcag cccacaggcg cacccacagc
     1021 ggcgagaagc cctacccctg cccgcagtgc ggccgccgct ttggccagaa gtcagccgtg
     1081 gccaaacacc agtgggttca tcggcccggg gccgggggcc acaggggccg ggtcgccggg
     1141 cgtctgtctg tgaccctgac ccctggccac ggagacctgg acccgcccgt gggcttccag
     1201 ctgtacccgg agatattcca ggagtgtggg tagtaatgaa ctagtccgta acttgaaagt
     1261 atttcgattt cttggcttta tatatcttgt ggaaaggacg aggatactgc cttgctactc
     1321 gtccg
//
