LOCUS       sequence-28067-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  TITLE     pSVOA-delta5-mcfos-luc
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSFG-tcLuc+ITEA-
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(113..137)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(134..172)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(205..223,224..246,247..265,266..288,289..307,308..330,
                     331..349,350..372,373..391,392..414,415..433,434..456,
                     457..475)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(534..550)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 cggaagaatg gcgccgggcc tttctttatg tttttggcgt cttccatggc aggtcggatc
       61 ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac
      121 ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccta cctcgacccg
      181 ggtaccgagc tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
      241 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
      301 atagggactg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc
      361 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
      421 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt
      481 aaactcgagg gctagaggat ggtccacccc cggggtcggc agccttcacg tgggcggcgt
      541 gtatccaagc tgcgatgccg tctactttga gggcggtggg ggtggtcagc aggactgtgt
      601 aaggtccttt ccagcgaggt tctaggttct tagtctggtg tcggcggacc cacactgtgt
      661 cgccgactcg gtaagggtga ggtaccaccg gtcggtccag ttgttcttgg taggccgccg
      721 ccagaggtct ccagacttcg tgctggacta agtagagagc ctgtaagtga gcttggagag
      781 agggctgtta gtaactct
//
