LOCUS       sequence-280685-        1544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1774
FEATURES             Location/Qualifiers
     source          1..1544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccactgt ccctggcctc cagcccctgc caaccttgga gcaggacctg
       61 gaacaggaag agatcctgat tgtgaaggtg gaggaggact tctgcttgga agaggagccc
      121 tccgtggaga cggaggaccc cagccctgag actttccgcc agctcttccg gctcttctgc
      181 taccaggagg tggctgggcc ccgggaggcc ctgagccgcc tctgggagct gtgctgccgc
      241 tggctgcggc cggagctgcg caccaaggag cagatcctgg agctgctggt gctggagcag
      301 ttcctgactg tgctgccggg ggagatccag gctcgggtac gcgagcagca gccggagagc
      361 ggtgaggagg ccgtggtcct tgtggaaggg ctgcagcgga agcccaggaa acacaggcag
      421 cggggctcag agctgctttc tgatgacgag gtgcccctcg ggataggggg acagttctta
      481 aaacaccagg cagaggctca gccagaggat ctgtccctgg aggaagaggc tcgattctcc
      541 agccagcagc ccccagccca gctgagccac aggccacaga ggggcccgct gttgtggcca
      601 gagagaggcc ctccagctcc ccggcatcag gagatggcgt cagcctcgcc cttcctttcg
      661 gcctggtccc aggtgcccgt gaacttggag gacgtggctg tatacctttc tggggaggag
      721 ccaagatgca tggacccagc tcagcgggac gcgccgctgg agaatgaagg acctgggatc
      781 cagttggagg acggcggtga tggcagggag gatgccccgt tgagaatgga gtggtaccga
      841 gtgctctcgg cacgatgcca ggggcctggc cacccgctcc caggtcagag gccagcccca
      901 gtcaggggct tggtcaggcc tgatcagcca agaggcggcc ccccaccagg aagacgggct
      961 tcccatgggg ctgacaagcc gtacacctgc cccgaatgtg gcaaaggctt cagcaagacg
     1021 tcccacttga ccaagcacca gcgcacacac acgggcgagc ggccttacaa gtgcctagtc
     1081 tgtgggaagg gctttagcga ccgctccaac ttcagcacgc accagagggt gcacacaggc
     1141 gagaagccct acccgtgccc cgagtgtggg aagcgcttca gccagagctc cagcctggtc
     1201 atccaccgca ggacacacag cggggagcgg ccctatgcct gcacccagtg cgggaagcgc
     1261 ttcaacaaca gctcgcactt cagcgcccac cgccggacgc acacaggtga gaagccctac
     1321 acctgcccgg cctgtggccg gggcttccgc cggggcaccg acctgcacaa gcaccagcgg
     1381 acccacatgg gggcaggctc cttgccgacg ctccagccgg tggctcctgg aggccccggt
     1441 gccaaagcct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1501 atatcttgtg gaaaggacga accgaggatc aaggagcgct cttt
//
