LOCUS       sequence-280688-        1991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1776
FEATURES             Location/Qualifiers
     source          1..1991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaatttag catcaagcag agtccccttt ctgttcagag tgttgtaaag
       61 tgcataaaga tgaagcaggc accagaaatc ctcggcagtg ccaacgggaa gactccgagc
      121 tgcgaggtga accgcgagtg ttctgtgttc ctcagcaaag cccagctctc cagtagcctg
      181 caggaggggg tcatgcagaa gtttaacggc cacgacgccc tgccctttat tccagccgac
      241 aagctgaaag atcttacttc ccgggtgttt aatggagaac ccggcgcaca cgatgccaaa
      301 ctgcgttttg agtcccagga aatgaaaggg attgggacac cccctaacac tacccctatc
      361 aaaaatggct ctccagaaat taagctgaaa atcaccaaaa catacatgaa tgggaagcct
      421 ctctttgaat cttccatttg tggtgacagt gctgctgatg tgtctcagtc agaagaaaat
      481 ggacaaaaac cagaaaacaa ggcgagaagg aacaggaaga ggagcataaa atatgactcc
      541 ttgctggagc agggccttgt cgaagcagct cttgtgtcta agatctcaag tccttcagat
      601 aaaaagattc cagctaagaa agagtcttgt ccaaacactg gaagagacaa agaccacctg
      661 ttgaaataca acgttggtga tttggtgtgg tccaaagtgt cgggttaccc ttggtggcct
      721 tgcatggttt ctgcagatcc actccttcac agctatacca aacttaaagg tcagaaaaag
      781 agtgcacgcc agtatcacgt acagttcttt ggtgacgccc cagaaagagc ttggatattt
      841 gagaagagcc tcgtagcttt tgaaggagaa ggacagtttg aaaaattatg ccaggaaagt
      901 gccaagcagg cacccacgaa agctgagaaa attaagctat tgaaaccaat ttcagggaaa
      961 ttgagggccc agtgggaaat gggcattgtt caagcagaag aagctgcaag catgtcagtg
     1021 gaggagcgga aagccaagtt cacctttctc tatgtggggg accagcttca tctcaaccct
     1081 caagtagcca aggaggctgg cattgctgca gagtctttgg gagaaatggc agaatcctca
     1141 ggagtcagtg aagaagctgc tgaaaacccc aagtctgtga gagaagagtg cattcccatg
     1201 aagagaaggc ggagggccaa actgtgtagc tctgcagaga ccctggagag tcaccccgac
     1261 atagggaaga gtactcctca aaagacggca gaggctgacc ccagaagagg agtagggtct
     1321 cctcctggga ggaagaagac cacagtctcc atgccacgaa gcaggaaggg agatgcagca
     1381 tcccagtttt tggtcttctg tcaaaaacac agggatgagg tggtagctga gcacccagat
     1441 gcttcaggtg aggagattga agagctgctc aggtcacagt ggagtctgct gagtgagaag
     1501 cagagagcac gctacaacac caagtttgcc ctggtggccc ctgtccaggc tgaagaagac
     1561 tctggtaatg taaatgggaa aaaaagaaac cacacaaaga ggatacagga ccctacagaa
     1621 gatgctgaag ctgaggacac acccaggaaa agactcagga cggacaagca cagtcttcgg
     1681 aagagagaca caatcactga caaaacggcc agaacaagct cttacaaggc catggaggca
     1741 gcctcctcgc tcaagagcca ggcagcaacg aaaaatctgt ctgatgcatg taaaccactg
     1801 aagaagcgaa atcgggcttc cacggcagca tcttcagctc ttgggtttag caaaagttca
     1861 tctccttctg catccttaac tgagaatgag gtaaaatagt aatgaactag tccgtaactt
     1921 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacag tactccatct
     1981 gcgaggatac c
//
