LOCUS       sequence-280694-        2963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1779
FEATURES             Location/Qualifiers
     source          1..2963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagaaag tagcagtgtt gccatgttgg tgccagatat tggggaacag
       61 gaagctatac tgactgctga aagtatcatc agtccttcat tggaaattga tgaacaaaga
      121 aaaactaaac cagatccatt aatccatgtt atccagaagt taagcaagat agtggaaaat
      181 gaaaagtcac aaaaatgtct tttaattggg aagaaacgcc cacgttcaag tgctgcaaca
      241 cactctcttg aaacccaaga actttgtgag attccggcta aagtaatcca gtcacctgct
      301 gctgatacta gaagggctga gatgtcacaa acaaatttta cccctgacac tcttgcccag
      361 aatgaaggga aggctatgtc ttatcagtgt agcctttgta agtttctatc atcatccttt
      421 tccgtgttaa aagatcatat taagcaacat ggtcagcaaa atgaagtgat actgatgtgc
      481 tcagagtgcc atattacatc tagaagccag gaggaacttg aagcccacgt ggtgaatgac
      541 catgacaatg atgccaatat ccacacccaa tccaaagccc aacagtgcgt aagcccctcc
      601 agctctttgt gtcggaaaac cacagaaaga aatgaaacca ttccagatat cccagtaagt
      661 gtggacaatc tacagactca tactgtccaa actgcatctg tggcagaaat gggtaggagg
      721 aaatggtatg catacgaaca gtacggcatg tatcgatgct tgttttgtag ttatacttgt
      781 ggccagcaga gaatgttgaa aacacacgct tggaaacatg ctggggaggt tgattgctcc
      841 tatccaatct ttgaaaatga aaatgaaccc ctaggcctgc tggattcttc agcagctgct
      901 gcgcctggtg gggtcgatgc agtcgtcatt gctattggag agagtgaact gagtatccac
      961 aatgggccat cagtgcaagt gcagatttgc agctcagaac agttatcatc ttcatctcct
     1021 ttagaacaga gtgcagaaag aggagtacac ctaagtcagt cagttaccct ggaccccaat
     1081 gaggaagaaa tgctagaagt gatttctgat gcagaggaga atctgattcc tgatagcctg
     1141 cttacatcag cacagaaaat catcagcagc agccccaata aaaaagggca tgttaacgtg
     1201 atagtggagc gattgccaag tgctgaagaa accctttcac agaagcgctt cctcatgaac
     1261 actgaaatgg aagaagggaa ggacctgagc ctgacagaag ctcagattgg gcgcgaagga
     1321 atggatgatg tttatcgtgc tgataaatgt actgttgata ttgggggatt gatcataggc
     1381 tggagcagtt cagagaaaaa agacgagtta atgaataaag gcctggctac tgatgagaat
     1441 gccccaccag gccggagaag gacaaattct gagtctcttc gattacactc attagctgca
     1501 gaagcccttg tcacaatgcc tataagagct gcagagttga caagagccaa cctggggcac
     1561 tatggagata taaacctttt agatccagat actagtcaaa ggcaagtaga tagtacattg
     1621 gcagcgtact caaaaatgat gtcgccactt aaaaactctt cagatggatt aactagtctt
     1681 aaccaaagca actccacctt ggtagcactc ccagagggta ggcaggaatt gtcagatggg
     1741 caggttaaga caggcatcag catgtcctta ctcaccgtca ttgaaaaatt gagagaaagg
     1801 acagaccaaa acgcttcaga cgatgacatt ttgaaagagt tgcaggacaa cgcccagtgc
     1861 caacccaaca gcgatacaag tttgtccgga aacaatgtgg tggaatacat cccgaatgct
     1921 gaacgaccct accgttgccg cctgtgtcac tacacaagtg gcaacaaggg ctacatcaag
     1981 cagcacttac gagtccatcg acagagacag ccttatcagt gtcctatctg cgagcacata
     2041 gcggacaaca gcaaagattt ggagagtcac atgatccacc actgtaagac aagaatatac
     2101 cagtgcaagc agtgtgaaga atccttccat tataagagtc aattgaggaa ccatgagaga
     2161 gaacagcaca gtcttccaga taccttgtca atagcaactt ctaatgagcc aagaatttcc
     2221 agtgatacag ctgatggaaa atgtgtccag gaagggaata agtcttcagt ccagaaacaa
     2281 tatagatgtg atgtgtgtga ttatacaagt acaacatatg ttggtgtcag aaaccacagg
     2341 cgaatccata actctgataa gccgtacaga tgctctctgt gtgggtatgt gtgtagccat
     2401 cctccttctt tgaagtctca tatgtggaaa catgcaagtg accaaaatta caactacgaa
     2461 caagtaaaca aggctattaa cgacgcgatt tcacaaagtg gcagagttct ggggaaatcc
     2521 cctggaaaga ctcaattaaa gagcagtgaa gagagtgcag atcccgtcac tggaagttca
     2581 gaaaatgcag tgtcatcttc agaactgatg tcccagactc ccagtgaagt tctgggtacc
     2641 aacgagaatg agaaactgag ccctacaagt aatacctcat atagtttaga aaaaatctcc
     2701 agtctggccc ctcctagcat ggagtactgc gttttactct tctgctgttg tatttgtggt
     2761 tttgaatcaa ccagcaaaga aaacctcttg gatcatatga aagagcacga gggtgaaatt
     2821 gtaaacatca tcctgaataa ggaccacaat acagctctaa acacaaatta gtaatgaact
     2881 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     2941 gtacctctga agtcaaggac agg
//
