LOCUS       sequence-280724-        1241 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1833
FEATURES             Location/Qualifiers
     source          1..1241
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaccagg aaacctctgg catatgagaa ataactttct ctttggttca
       61 agatgttgga tgactcgatt ttcagcagaa aacatcttca aatcagtttc atttaggctt
      121 tttggtgtga agtgtcataa tacagacagt gagcctttga aaaatgagga cctactgaaa
      181 aacttactta ctatgggagt agatattgac atggcaagga aacgacagcc tggagttttt
      241 cataggatga ttaccaatga gcaggacctg aagatgttcc ttctttccaa aggagctagc
      301 aaagaagtga tcgctagcat catatcaaga tatccacgag caataacacg tactcccgag
      361 aatctttcaa aacggtggga tctgtggaga aagattgtga catcagacct tgaaattgta
      421 aatattttgg aacgttctcc tgaatccttt tttcggtcca ataacaacct aaacttagag
      481 aataatataa agttcctcta ctcagttgga ttgacccgta aatgcctttg tcgattgttg
      541 accaatgccc ctcgtacctt ctccaatagt cttgatctga ataaacagat ggttgaattt
      601 ttgcaggcag ccggtttgtc attgggtcac aatgatcccg cagattttgt cagaaagata
      661 atttttaaaa acccttttat cttaattcag agcaccaagc gggtgaaagc taacattgaa
      721 ttcttacggt caactttcaa tttgaacagt gaggaactgc tggttctgat atgtggtcca
      781 ggagctgaaa tcctagacct ttccaatgac tatgccagaa gaagctacgc aaacatcaaa
      841 gagaagctgt tttctcttgg atgtactgaa gaagaggtac agaagtttgt cttaagctat
      901 ccagatgtga tcttcttggc agagaaaaag tttaatgata aaatagactg cctcatggaa
      961 gaaaacatta gcatttcaca aataatcgaa aatcctcggg ttctggattc aagcataagt
     1021 actttaaaaa gtcgaatcaa agaattggta aatgctggct gtaacttgag tactttaaac
     1081 atcactcttc tatcttggag taaaaaaaga tatgaagcta aattgaaaaa gttaagcaga
     1141 tttgcctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1201 tcttgtggaa aggacgagtc tgtgagtgag ctgctatcgg a
//
