LOCUS       sequence-280733-        2342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1838
FEATURES             Location/Qualifiers
     source          1..2342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggccagac tgggaagaaa tctgagaagg gaccagtttg ttggcggaag
       61 cgtgtaaaat cagagtacat gcgactgaga cagctcaaga ggttcagacg agctgatgaa
      121 gtaaagagta tgtttagttc caatcgtcag aaaattttgg aaagaacgga aatcttaaac
      181 caagaatgga aacagcgaag gatacagcct gtgcacatcc tgacttctgt gagctcattg
      241 cgcgggacta gggagtgttc ggtgaccagt gacttggatt ttccaacaca agtcatccca
      301 ttaaagactc tgaatgcagt tgcttcagta cccataatgt attcttggtc tcccctacag
      361 cagaatttta tggtggaaga tgaaactgtt ttacataaca ttccttatat gggagatgaa
      421 gttttagatc aggatggtac tttcattgaa gaactaataa aaaattatga tgggaaagta
      481 cacggggata gagaatgtgg gtttataaat gatgaaattt ttgtggagtt ggtgaatgcc
      541 cttggtcaat ataatgatga tgacgatgat gatgatggag acgatcctga agaaagagaa
      601 gaaaagcaga aagatctgga ggatcaccga gatgataaag aaagccgccc acctcggaaa
      661 tttccttctg ataaaatttt tgaagccatt tcctcaatgt ttccagataa gggcacagca
      721 gaagaactaa aggaaaaata taaagaactc accgaacagc agctcccagg cgcacttcct
      781 cctgaatgta cccccaacat agatggacca aatgctaaat ctgttcagag agagcaaagc
      841 ttacactcct ttcatacgct tttctgtagg cgatgtttta aatatgactg cttcctacat
      901 ccttttcatg caacacccaa cacttataag cggaagaaca cagaaacagc tctagacaac
      961 aaaccttgtg gaccacagtg ttaccagcat ttggagggag caaaggagtt tgctgctgct
     1021 ctcaccgctg agcggataaa gaccccacca aaacgtccag gaggccgcag aagaggacgg
     1081 cttcccaata acagtagcag gcccagcacc cccaccatta atgtgctgga atcaaaggat
     1141 acagacagtg atagggaagc agggactgaa acggggggag agaacaatga taaagaagaa
     1201 gaagagaaga aagatgaaac ttcgagctcc tctgaagcaa attctcggtg tcaaacacca
     1261 ataaagatga agccaaatat tgaacctcct gagaatgtgg agtggagtgg tgctgaagcc
     1321 tcaatgttta gagtcctcat tggcacttac tatgacaatt tctgtgccat tgctaggtta
     1381 attgggacca aaacatgtag acaggtgtat gagtttagag tcaaagaatc tagcatcata
     1441 gctccagctc ccgctgagga tgtggatact cctccaagga aaaagaagag gaaacaccgg
     1501 ttgtgggctg cacactgcag aaagatacag ctgaaaaagg acggctcctc taaccatgtt
     1561 tacaactatc aaccctgtga tcatccacgg cagccttgtg acagttcgtg cccttgtgtg
     1621 atagcacaaa atttttgtga aaagttttgt caatgtagtt cagagtgtca aaaccgcttt
     1681 ccgggatgcc gctgcaaagc acagtgcaac accaagcagt gcccgtgcta cctggctgtc
     1741 cgagagtgtg accctgacct ctgtcttact tgtggagccg ctgaccattg ggacagtaaa
     1801 aatgtgtcct gcaagaactg cagtattcag cggggctcca aaaagcatct attgctggca
     1861 ccatctgacg tggcaggctg ggggattttt atcaaagatc ctgtgcagaa aaatgaattc
     1921 atctcagaat actgtggaga gattatttct caagatgaag ctgacagaag agggaaagtg
     1981 tatgataaat acatgtgcag ctttctgttc aacttgaaca atgattttgt ggtggatgca
     2041 acccgcaagg gtaacaaaat tcgttttgca aatcattcgg taaatccaaa ctgctatgca
     2101 aaagttatga tggttaacgg tgatcacagg ataggtattt ttgccaagag agccatccag
     2161 actggcgaag agctgttttt tgattacaga tacagccagg ctgatgccct gaagtatgtc
     2221 ggcatcgaaa gagaaatgga aatcccttag taatgaacta gtccgtaact tgaaagtatt
     2281 tcgatttctt ggctttatat atcttgtgga aaggacgact atccggtgcg gaactgccac
     2341 ag
//
