LOCUS       sequence-280736-        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1840
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             133..153
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 accgccacca tggaactaaa aactttatgg gaaaacaaac taatgcagtc cagggcagta
       61 gatggatttc attcagaaga gcagcagctt ctactgcaag ttcaacagca gcatcaaccc
      121 cagcagcagc agcatcacca ccatcaccat catcagcaag ctcagcctca gcagacagta
      181 cctcagcaag cgcagaccca gcaggttctt attcctgcat cacagcaagc cacagcacca
      241 caagttattg ttccagattc taagttgata cagcatatga atgcatcaaa catgagtgct
      301 gctgctacag ctgctacctt agcactccct gcaggtgtga ctcctgttca gcagatatta
      361 acaaattcag gccagcttct tcaggtggtc agagcagcca atggtgccca atatatcttt
      421 cagcctcagc agtcagtggt tctacaacaa caggttatac cacaaatgca gcctggtgga
      481 gtacaagctc ctgttataca gcaggtgctg gctcctcttc ctggagggat ttcaccacag
      541 acaggtgtca tcatccagcc tcagcaaatc ttatttacag gaaataagac tcaagttata
      601 cctacgacag tggcagcacc tacaccagcc caagcacaga taactgcaac tggccagcag
      661 caaccgcagg cccagcctgc tcaaacacaa gctccattgg tcttacaagt tgatggaact
      721 ggggatacat catctgaaga agatgaagat gaagaagaag actatgatga tgatgaggag
      781 gaagacaaag agaaagatgg agctgaagat gggcaggtgg aagaagagcc cctcaatagt
      841 gaagatgatg tgagtgatga ggaaggacag gaactctttg acacagaaaa tgttgttgta
      901 tgccaatatg ataagataca cagaagtaaa aacaaatgga aatttcatct caaggatggc
      961 attatgaatc ttaatggaag agattatata ttttccaaag ccattggaga tgcagaatgg
     1021 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1081 ggaaaggacg aactacggct gcagttgcaa cggca
//
