LOCUS       sequence-280748-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1850
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgatcg agaggatctg gtgtaccagg cgaagctggc cgagcaggct
       61 gagcgatacg acgaaatggt ggagtcaatg aagaaagtag cagggatgga tgtggagctg
      121 acagttgaag aaagaaacct cctatctgtt gcatataaga atgtgattgg agctagaaga
      181 gcctcctgga gaataatcag cagcattgaa cagaaagaag aaaacaaggg aggagaagac
      241 aagctaaaaa tgattcggga atatcggcaa atggttgaga ctgagctaaa gttaatctgt
      301 tgtgacattc tggatgtact ggacaaacac ctcattccag cagctaacac tggcgagtcc
      361 aaggttttct attataaaat gaaaggggac taccacaggt atctggcaga atttgccaca
      421 ggaaacgaca ggaaggaggc tgcggagaac agcctagtgg cttataaagc tgctagtgat
      481 attgcaatga cagaacttcc accaacgcat cctattcgct taggtcttgc tctcaatttt
      541 tccgtattct actacgaaat tcttaattcc cctgaccgtg cctgcaggtt ggcaaaagca
      601 gcttttgatg atgcaattgc agaactggat acgctgagtg aagaaagcta taaggactct
      661 acacttatca tgcagttgtt acgtgataat ctgacactat ggacttcaga catgcagggt
      721 gacggtgaag agcagaataa agaagcgctg caggacgtgg aagacgaaaa tcagtagtaa
      781 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      841 gacgatgtcg gctccatgac gtcttaaac
//
