LOCUS       sequence-280754-        2036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1853
FEATURES             Location/Qualifiers
     source          1..2036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaaagcc cagccacagc agctatgtcc ttcagcagct aaacaaccaa
       61 agagaatggg gttttctctg tgactgctgt attgcaattg atgacattta ctttcaagca
      121 cacaaggcag ttctagctgc ctgtagctcc tattttagaa tgtttttcat gaaccatcag
      181 catagtactg cacaactgaa tctcagcaac atgaaaatta gtgcagaatg ttttgatctc
      241 attttgcagt ttatgtattt aggaaaaatt atgacagctc cctccagttt tgagcagttt
      301 aaagtggcaa tgaactacct acagctatac aatgttcctg actgtttaga agacatccag
      361 gatgcagatt gttctagttc aaaatgttcc tcttctgctt ccagcaaaca gaacagcaaa
      421 atgatatttg gggtaagaat gtatgaagat actgtggctc gaaatggcaa tgaagccaac
      481 aggtggtgtg cagagccaag ttcaacggta aatacaccac ataatagaga ggctgatgaa
      541 gagtctttac aattaggtaa ttttcctgag ccactatttg atgtatgtaa aaaaagttcc
      601 gtgtccaaat tatctactcc aaaagaacgt gtgtcaagac gctttgggcg gagttttacc
      661 tgtgatagct gtggatttgg ctttagctgt gaaaaattat tagatgagca tgtgctaacc
      721 tgtactaaca gacatttata ccaaaacaca agatcttacc atagaatagt agatattaga
      781 gatggaaaag acagtaacat caaagctgaa tttggtgaaa aagattcttc caaaacattt
      841 tctgcacaga cggacaaata cagaggagac acaagccagg ctgctgatga ttcagcttca
      901 accactggaa gcagaaaaag tagcacagtg gagtctgaaa tagcaagcga agagaaaagc
      961 agagctgctg agaggaaaag gattattatt aagatggagc cagaagatat tcctacagat
     1021 gaactgaaag actttaacat tattaaagtt actgataaag actgtaatga atccactgac
     1081 aatgatgaat tagaagatga acctgaagag ccattttata gatactatgt tgaagaagat
     1141 gtcagcataa aaaaaagtgg taggaaaact ctaaaacctc gaatgtcagt aagtgctgat
     1201 gaaagaggtg gtttagagaa tatgaggccc cctaacaaca gcagtccagt acaagaggat
     1261 gctgaaaatg catcttgtga gctgtgtgga cttacaataa ccgaggagga cctgtcatct
     1321 cattacttag ccaaacacat tgaaaatatc tgtgcatgtg gtaaatgtgg acaaatactt
     1381 gtaaagggta ggcagcttca ggaacatgct caacgatgtg gcgagcccca agatctgacc
     1441 atgaatgggt taggaaatac tgaggagaaa atggacttgg aagagaatcc tgatgagcag
     1501 tccgaaataa gagatatgtt tgttgaaatg ctggatgatt ttagggacaa tcattaccag
     1561 ataaacagta tccaaaaaaa gcagttattt aaacattctg cctgcccttt tcgatgtcct
     1621 aattgtggcc agcgttttga aactgaaaat ctagtggttg aacatatgtc tagctgctta
     1681 gatcaagata tgtttaagag tgccatcatg gaagaaaatg aaagagatca cagacgaaag
     1741 catttttgta atctgtgtgg aaaaggattt tatcagcggt gtcacttaag agaacactat
     1801 actgttcata ctaaggaaaa acagtttgtt tgtcaaacat gtggaaagca gtttttaaga
     1861 gagcgtcagt tgcgactgca caatgatatg cacaaaggca tggccagtgg tgaaataggg
     1921 ccttctaaac ctgtggagaa gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1981 tcttggcttt atatatcttg tggaaaggac gatgtattta cagcggaagc gccaag
//
