LOCUS       sequence-280757-        2135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1854
FEATURES             Location/Qualifiers
     source          1..2135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggattttcc tggtcacttt gaacaaatct tccagcagct gaactaccag
       61 agacttcatg gccagctctg tgattgtgtc attgtagtgg ggaatagaca ctttaaagcc
      121 caccgctccg tgctggcagc atgcagcacg catttccgag ccctgttctc agtggcagaa
      181 ggagatcaga ccatgaacat gatccagctg gatagcgagg tggtgacagc agaggccttt
      241 gctgcactga ttgacatgat gtatacctcc accctcatgc tgggggagag caatgtaatg
      301 gatgtcttat tggcagcctc tcacctgcat ttgaactctg ttgttaaggc atgtaaacat
      361 tacttaacga caaggacgct gcccatgtct ccccccagtg agcgcgttca ggagcagagc
      421 gcccgcatgc agcgctcctt tatgctacag cagctgggac taagcatcgt gagctcagcc
      481 ctcaattcca gccagaatgg cgaggagcag ccagccccca tgagctcttc catgcgcagt
      541 aacctggatc agcgcacgcc cttccccatg agacgccttc ataagcgcaa gcagtctgca
      601 gaggagcggg ccaggcagcg cctccgaccc tccatagatg agtctgccat ttcagatgtt
      661 acaccggaga atgggccttc aggggttcat tctcgggagg agttcttttc accagattct
      721 ctgaaaattg tggataatcc taaagctgat ggaatgactg ataaccagga agatagtgcg
      781 atcatgtttg atcagtcttt tggcactcaa gaagatgccc aggtgcccag ccagtctgat
      841 aacagtgctg gcaacatggc acagttgtcc atggcctctc gtgcaactca ggttgagact
      901 agttttgatc aggaagctgc acctgagaaa agtagttttc agtgtgaaaa ccctgaggtt
      961 ggccttggtg agaaggagca catgagagtg gtggttaaat ctgagcccct gagctcacct
     1021 gagcctcagg atgaagtgag cgatgtgacc tcacaagcag aaggcagcga gtctgtggaa
     1081 gtggaaggag ttgtggtcag tgccgagaag atagacctca gccctgaaag cagtgatcgg
     1141 agtttttcag atccccagtc tagcacagac agggtaggtg atatccatat tttggaagtc
     1201 acaaataacc tagagcataa gtccactttt agtatttcga attttcttaa caagagcaga
     1261 ggaaataact ttactgcaaa tcagaacaat gatgataata ttccaaacac cactagtgac
     1321 tgcaggctgg agagtgaggc cccctatttg ttgagtccag aggctgggcc tgcaggtggg
     1381 ccctcctctg cccctggctc ccatgtagag aacccattta gtgaacctgc agactcccac
     1441 ttcgtcaggc ctatgcagga ggtgatgggc ctgccgtgtg tgcagacttc aggctaccaa
     1501 ggaggagaac agtttgggat ggacttttcc aggtctggtt tgggcctcca ctcctccttc
     1561 tccagggtaa tgataggttc cccaagggga ggagccagta actttcctta ctaccgccgc
     1621 atagctccca aaatgccagt tgtaacttcc gtcaggagct cacagatccc agaaaactct
     1681 accagttctc agctaatgat gaatggagct acgtcctcat ttgaaaatgg ccatccttcc
     1741 cagcctggcc ctccacagtt gaccagggca tctgcagatg ttctgtcaaa gtgcaagaag
     1801 gccttatcag agcacaatgt tttggttgta gagggagctc gcaagtatgc ctgcaaaatc
     1861 tgctgcaaaa cttttctgac tttgacagat tgcaagaagc acatccgtgt tcacacaggt
     1921 gaaaagcctt acgcctgcct gaagtgtggc aagaggttta gtcagtccag ccacctgtat
     1981 aagcactcaa agactacctg cctgcgctgg cagagcagca atcttcccag cactttgctc
     2041 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2101 ggaaaggacg acaatgacag tgaggcacta gtctc
//
