LOCUS       sequence-280763-        1709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1857
FEATURES             Location/Qualifiers
     source          1..1709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacaacta cagcacaggc tacgacgtca agccaccttg cttgtaccaa
       61 atgcccctgt ccggacagca gtcctccatt aaggtagaag acattcagat gcacaactac
      121 cagcaacaca gccacctgcc cccccagtct gaggagatga tgccgcactc cgggtcggtt
      181 tactacaagc cctcctcgcc cccgacgccc accaccccgg gcttccaggt gcagcacagc
      241 cccatgtggg acgacccggg atctctccac aacttccacc agaactacgt ggccactacg
      301 cacatgatcg agcagaggaa aacgccagtc tcccgcctct ccctcttctc ctttaagcaa
      361 tcgccccctg gcaccccggt gtctagttgc cagatgcgct tcgacgggcc cctgcacgtc
      421 cccatgaacc cggagcccgc cggcagccac cacgtggtgg acgggcagac cttcgctgtg
      481 cccaacccca ttcgcaagcc cgcgtccatg ggcttcccgg gcctgcagat cggccacgcg
      541 tctcagctgc tcgacacgca ggtgccctca ccgccgtcgc ggggctcccc ctccaacgag
      601 gggctgtgcg ctgtgtgtgg ggacaacgcg gcctgccaac actacggcgt gcgcacctgt
      661 gagggctgca aaggcttctt taagcgcaca gtgcaaaaaa atgcaaaata cgtgtgttta
      721 gcaaataaaa actgcccagt ggacaagcgt cgccggaatc gctgtcagta ctgccgattt
      781 cagaagtgcc tggctgttgg gatggtcaaa gaagtggttc gcacagacag tttaaaaggc
      841 cggagaggtc gtttgccctc gaaaccgaag agcccacagg agccctctcc cccttcgccc
      901 ccggtgagtc tgatcagtgc cctcgtcagg gcccatgtcg actccaaccc ggctatgacc
      961 agcctggact attccaggtt ccaggcgaac cctgactatc aaatgagtgg agatgacacc
     1021 cagcatatcc agcaattcta tgatctcctg actggctcca tggagatcat ccggggctgg
     1081 gcagagaaga tccctggctt cgcagacctg cccaaagccg accaagacct gctttttgaa
     1141 tcagctttct tagaactgtt tgtccttcga ttagcataca ggtccaaccc agtggagggt
     1201 aaactcatct tttgcaatgg ggtggtcttg cacaggttgc aatgcgttcg tggctttggg
     1261 gaatggattg attccattgt tgaattctcc tccaacttgc agaatatgaa catcgacatt
     1321 tctgccttct cctgcattgc tgccctggct atggtcacag agagacacgg gctcaaggaa
     1381 cccaagagag tggaagaact gcaaaacaag attgtaaatt gtctcaaaga ccacgtgact
     1441 ttcaacaatg gggggttgaa ccgccccaat tatttgtcca aactgttggg gaagctccca
     1501 gaacttcgta ccctttgcac acaggggcta cagcgcattt tctacctgaa attggaagac
     1561 ttggtgccac cgccagcaat aattgacaaa cttttcctgg acactttacc tttctagtaa
     1621 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1681 gacgaattgg gatgggctcg aggctagca
//
