LOCUS       sequence-280775-        2213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1870
FEATURES             Location/Qualifiers
     source          1..2213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggacgg aggagcagcg agtcaagatg agagttcagc cgcggcggca
       61 gcagcagcag actcaagaat gaacaatccg tcagaaacca gtaaaccatc tatggagagt
      121 ggagatggca acacaggcac acaaaccaat ggtctggact ttcagaagca gcctgtgcct
      181 gtaggaggag caatctcaac agcccaggcg caggctttcc ttggacatct ccatcaggtc
      241 caactcgctg gaacaagttt acaggctgct gctcagtctt taaatgtaca gtctaaatct
      301 aatgaagaat cgggggattc gcagcagcca agccagcctt cccagcagcc ttcagtgcag
      361 gcagccattc cccagaccca gcttatgcta gctggaggac agataactgg ggatcttcaa
      421 caactgcaac agcttcaaca gcagaatctc aacctgcaac agtttgtgtt ggtgcatcca
      481 accaccaatt tgcagccagc gcagtttatc atctcacaga cgccccaggg ccagcagggt
      541 ctcctgcaag cgcaaaatct tctaacgcaa ctacctcagc aaagccaagc caacctccta
      601 cagtcgcagc caagcatcac cctcacctcc cagccagcaa ccccaacacg cacaatagca
      661 gcaaccccaa ttcagacact tccacagagc cagtcaacac caaagcgaat tgatactccc
      721 agcttggagg agcccagtga ccttgaggag cttgagcagt ttgccaagac cttcaaacaa
      781 agacgaatca aacttggatt cactcagggt gatgttgggc tcgctatggg gaaactatat
      841 ggaaatgact tcagccaaac taccatctct cgatttgaag ccttgaacct cagctttaag
      901 aacatgtgca agttgaagcc acttttagag aagtggctaa atgatgcaga gaacctctca
      961 tctgattcgt ccctctccag cccaagtgcc ctgaattctc caggaattga gggcttgagc
     1021 cgtaggagga agaaacgcac cagcatagag accaacatcc gtgtggcctt agagaagagt
     1081 ttcttggaga atcaaaagcc tacctcggaa gagatcacta tgattgctga tcagctcaat
     1141 atggaaaaag aggtgattcg tgtttggttc tgtaaccgcc gccagaaaga aaaaagaatc
     1201 aacccaccaa gcagtggtgg gaccagcagc tcacctatta aagcaatttt ccccagccca
     1261 acttcactgg tggcgaccac accaagcctt gtgactagca gtgcagcaac taccctcaca
     1321 gtcagccctg tcctccctct gaccagtgct gctgtgacga atctttcagt tacaggcact
     1381 tcagacacca cctccaacaa cacagcaacc gtgatttcca cagcgcctcc agcttcctca
     1441 gcagtcacgt ccccctctct gagtccctcc ccttctgcct cagcctccac ctccgaggca
     1501 tccagtgcca gtgagaccag cacaacacag accacctcca ctcctttgtc ctcccctctt
     1561 gggaccagcc aggtgatggt gacagcatca ggtttgcaaa cagcagcagc tgctgccctt
     1621 caaggagctg cacagttgcc agcaaatgcc agtcttgctg ccatggcagc tgctgcagga
     1681 ctaaacccaa gcctgatggc accctcacag tttgcggctg gaggtgcctt actcagtctg
     1741 aatccaggga ccctgagcgg tgctctcagc ccagctctaa tgagcaacag tacactggca
     1801 actattcaag ctcttgcttc tggtggctct cttccaataa catcacttga tgcaactggg
     1861 aacctggtat ttgccaatgc gggaggagcc cccaacatcg tgactgcccc tctgttcctg
     1921 aaccctcaga acctctctct gctcaccagc aaccctgtta gcttggtctc tgccgccgca
     1981 gcatctgcag ggaactctgc acctgtagcc agccttcacg ccacctccac ctctgctgag
     2041 tccatccaga actctctctt cacagtggcc tctgccagcg gggctgcgtc caccaccacc
     2101 accgcctcca aggcacagta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2161 tggctttata tatcttgtgg aaaggacgag cgccgatagt ctgcgtagcg gac
//
