LOCUS       sequence-280781-        2363 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1881
FEATURES             Location/Qualifiers
     source          1..2363
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagagagc cagacccgag ccgccgcctc agccgcgccc gttgcgtccc
       61 gctccgcccc cgctgccggt cgagggcacc tccttttggg cagcagccat ggagccccct
      121 ccgtcgtctc ccacactgag cgcggcagcc agtgcgacct tggcctcgtc gtgcggggag
      181 gcagtggcgt ccggcttaca gcccgcggtg cggcggctgc tgcaggtgaa gccagagcag
      241 gtgttgctgc taccacagcc tcaggcccag aacgaggaag ccgctgcctc gtccgcgcag
      301 gcgcggctgt tgcagttcag gcccgacctg cggctcctgc agccgccgac agcgtcagac
      361 ggcgccacct ccaggcccga gttgcacccg gtgcagcccc tggcgctgca tgtcaaggcc
      421 aagaagcaga agctggggcc cagcctggat cagtcagtgg ggcctcgagg ggccgtcgaa
      481 accggtccta gagcctccag ggtggtcaag ttggaaggcc ccgggccggc cctcggctac
      541 ttccgagggg acgagaaggg caagctggag gcggaggagg tcatgagaga ctcgatgcaa
      601 ggcggggcag gcaaaagccc ggcagccatc cgagaaggtg tgatcaaaac ggaggaaccc
      661 gagagactcc tcgaggactg caggctcggc gcggagcccg cgtccaatgg cctggttcat
      721 ggcagcgcgg aggtcatctt ggccccaacg tccggtgcct ttgggccgca ccagcaagac
      781 cttaggatcc ctttgacgct ccacacggtc ccccctgggg cccggatcca gtttcaggga
      841 gctccgcctt cagagctgat aagattgacc aaggtccccc tgacaccagt gcctactaaa
      901 atgcagtccc tactggagcc ttctgtaaaa attgaaacca aagatgtccc gctcaccgtg
      961 ttgccctcag atgcaggcat accagatact cccttcagta aggacagaaa tggtcatgtg
     1021 aagcgaccca tgaacgcatt tatggtttgg gcaaggatcc accgaccagc actagccaaa
     1081 gctaacccag cagccaacaa tgcagaaatc agtgtccagc ttgggttaga gtggaacaaa
     1141 cttagtgaag aacaaaagaa accctattac gatgaagcac aaaagattaa ggaaaagcac
     1201 agagaggaat ttcctggttg ggtttatcag cctcgtccag ggaagcgaaa acgattccct
     1261 ctaagtgttt ccaatgtatt ttctggtacc acacagaata tcatctctac aaatcctaca
     1321 acagtttatc cttaccgctc acctacgtac tctgtggtaa ttcccagcct acagaatccc
     1381 atcactcatc cagttggtga aacctcacct gctatccagc tgcccacacc tgcagtccag
     1441 agcccaagcc ctgtcacact tttccagccc agcgtctcca gtgctgctca ggtggctgtc
     1501 caggatccaa gtctacctgt ctatccagca ctcccacccc aacgctttac tgggccttcc
     1561 caaacagaca ctcatcagct gcattctgaa gccactcaca ctgtgaagca acccactcct
     1621 gtctctctag agagcgccaa caggatttca agtagtgcaa gtactgccca tgccagattt
     1681 gcaacttcga ccatccaacc tcctagggag tattccagcg tttccccttg tcccagaagt
     1741 gctccaatcc cccaggcttc tcccattcca cacccacatg tctaccagcc ccctcccctt
     1801 ggccatccag ccacactgtt cgggacacca ccaagattct cttttcatca cccttacttc
     1861 ctacccggac ctcactactt cccatcaagt acatgccctt acagtcggcc tccctttggc
     1921 tatggaaatt ttccgagttc aatgccagaa tgccttagtt attatgaaga caggtaccca
     1981 aaacatgagg gtatcttttc aactttaaat agagactatt cttttagaga ctactcaagt
     2041 gaatgcacac acagtgaaaa ttctcggagt tgtgagaaca tgaatggaac ttcttactat
     2101 aacagtcata gccacagtgg ggaagaaaac ttaaaccctg tgcctcagct ggacattgga
     2161 accttggaga atgtcttcac agccccgaca tcaactcctt ctagcatcca gcaagtcaat
     2221 gtcaccgaca gtgatgagga ggaagaagaa aaagtgctca gggatttata gtaatgaact
     2281 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     2341 ctcccacaga acataagtcg ttt
//
