LOCUS       sequence-280784-        1448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1883
FEATURES             Location/Qualifiers
     source          1..1448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaaatg ttgtagaggg aaggaacaaa gtctactgtg catccagcaa
       61 ggcataccag atactccctt cagtaaggac agaaatggtc atgtgaagcg acccatgaac
      121 gcatttatgg tttgggcaag gatccaccga ccagcactag ccaaagctaa cccagcagcc
      181 aacaatgcag aaatcagtgt ccagcttggg ttagagtgga acaaacttag tgaagaacaa
      241 aagaaaccct attacgatga agcacaaaag attaaggaaa agcacagaga ggaatttcct
      301 ggttgggttt atcagcctcg tccagggaag cgaaaacgat tccctctaag tgtttccaat
      361 gtattttctg gtaccacaca gaatatcatc tctacaaatc ctacaacagt ttatccttac
      421 cgctcaccta cgtactctgt ggtaattccc agcctacaga atcccatcac tcatccagtt
      481 ggtgaaacct cacctgctat ccagctgccc acacctgcag tccagagccc aagccctgtc
      541 acacttttcc agcccagcgt ctccagtgct gctcaggtgg ctgtccagga tccaagtcta
      601 cctgtctatc cagcactccc accccaacgc tttactgggc cttcccaaac agacactcat
      661 cagctgcatt ctgaagccac tcacactgtg aagcaaccca ctcctgtctc tctagagagc
      721 gccaacagga tttcaagtag tgcaagtact gcccatgcca gatttgcaac ttcgaccatc
      781 caacctccta gggagtattc cagcgtttcc ccttgtccca gaagtgctcc aatcccccag
      841 gcttctccca ttccacaccc acatgtctac cagccccctc cccttggcca tccagccaca
      901 ctgttcggga caccaccaag attctctttt catcaccctt acttcctacc cggacctcac
      961 tacttcccat caagtacatg cccttacagt cggcctccct ttggctatgg aaattttccg
     1021 agttcaatgc cagaatgcct tagttattat gaagacaggt acccaaaaca tgagggtatc
     1081 ttttcaactt taaatagaga ctattctttt agagactact caagtgaatg cacacacagt
     1141 gaaaattctc ggagttgtga gaacatgaat ggaacttctt actataacag tcatagccac
     1201 agtggggaag aaaacttaaa ccctgtgcct cagctggaca ttggaacctt ggagaatgtc
     1261 ttcacagccc cgacatcaac tccttctagc atccagcaag tcaatgtcac cgacagtgat
     1321 gaggaggaag aagaaaaagt gctcagggat ttatagtaat gaactagtcc gtaacttgaa
     1381 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatagcac atgtttgcgt
     1441 aaacctcg
//
