LOCUS       sequence-280787-        1913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1896
FEATURES             Location/Qualifiers
     source          1..1913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaaccat agaagaaatt gcacatcaaa ttattgaaca acagatggga
       61 gagattgtta cagagcagca aactgggcag aaaatccaga ttgtgacagc acttgatcat
      121 aatacccaag gcaagcagtt cattctgaca aatcacgacg gctctactcc aagcaaagtc
      181 attctggcca ggcaagattc cactccggga aaagttttcc ttacaactcc agatgcagca
      241 ggtgtcaacc agttattttt taccactcct gatctgtctg cacaacacct gcagctccta
      301 acagataatt ctccagacca aggaccaaat aaggtttttg atctttgcgt agtatgtgga
      361 gacaaagcat caggacgtca ttatggagca gtaacttgtg aaggctgcaa aggatttttt
      421 aaaagaagca tccgaaaaaa tttagtatat tcatgtcgag gatcaaagga ttgtattatt
      481 aataagcacc accgaaaccg ctgtcaatac tgcaggttac agagatgtat tgcgtttgga
      541 atgaagcaag actctgtcca atgtgaaaga aaacccattg aagtatcacg agaaaaatct
      601 tccaactgtg ccgcttcaac agaaaaaatc tatatccgaa aggaccttcg tagcccatta
      661 actgcaactc caacttttgt aacagatagt gaaagtacaa ggtcaacagg actgttagat
      721 tcaggaatgt tcatgaatat tcatccatct ggagtaaaaa ctgagtcagc tgtgctgatg
      781 acatcagata aggctgaatc atgtcaggga gatttaagta cattggccaa tgtggttaca
      841 tcattagcga atcttggaaa aactaaagat ctttctcaaa atagtaatga aatgtctatg
      901 attgaaagct taagcaatga tgatacctct ttgtgtgaat ttcaagaaat gcagaccaac
      961 ggtgatgttt caagggcatt tgacactctt gcaaaagcat tgaatcctgg agagagcaca
     1021 gcctgccaga gctcagtagc gggcatggaa ggaagtgtac acctaatcac tggagattca
     1081 agcataaatt acaccgaaaa agaggggcca cttctcagcg attcacatgt agctttcagg
     1141 ctcaccatgc cttctcctat gcctgagtac ctgaatgtgc actacattgg ggagtctgcc
     1201 tccagactgc tgttcttatc aatgcactgg gcactttcga ttccttcttt ccaggctcta
     1261 gggcaagaaa acagcatatc actggtgaaa gcttactgga atgaactttt tactcttggt
     1321 cttgcccagt gctggcaagt gatgaatgta gcaactatat tagcaacatt tgtcaattgt
     1381 cttcacaata gtcttcaaca agataaaatg tcaacagaaa gaagaaaatt attgatggag
     1441 cacatcttca aactacagga gttttgtaac agcatggtta aactctgcat tgatggatac
     1501 gaatatgcct acctgaaggc aatagtactc ttcagtccag atcatccaag cctagaaaac
     1561 atggaacaga tagagaaatt tcaggaaaag gcttatgtgg aattccaaga ttatataacc
     1621 aaaacatatc cagatgacac ctacaggtta tccagactac tactcagatt gccagcttta
     1681 agactgatga atgctaccat cactgaagaa ttgtttttca aaggtctcat tggcaatata
     1741 cgaattgaca gtgttatccc acatattttg aaaatggagc ctgcagatta taactctcaa
     1801 ataattggtc acagcattta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1861 tggctttata tatcttgtgg aaaggacgat ctggtcagaa agggtcacat ccg
//
