LOCUS       sequence-280811-        1535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1935
FEATURES             Location/Qualifiers
     source          1..1535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcaaag gtacgatgag ctgccccatt acggcgggat ggacggagta
       61 ggggttcccg cttccatgta cggagaccct cacgcgccgc ggccgatccc cccggttcac
      121 cacctgaacc acgggccgcc gctccacgcc acacagcact acggcgcgca cgccccgcac
      181 cccaatgtca tgccggccag tatgggatcc gctgtcaacg acgccttgaa gcgggacaag
      241 gacgcgatct atgggcaccc gttgtttcct ctgttagctc tggtctttga gaagtgcgag
      301 ctggcgacct gcactccccg ggaacctgga gtggctggcg gagacgtctg ctcctccgac
      361 tccttcaacg aggacatcgc ggtcttcgcc aagcaggttc gcgccgaaaa gccacttttt
      421 tcctcaaatc cagagctgga caatttgatg atacaagcaa tacaagtact aaggtttcat
      481 cttttggagt tagaaaaggt ccacgaactg tgcgataact tctgccaccg atacattagc
      541 tgtttgaagg ggaaaatgcc catcgacctc gtcattgatg aaagagacgg cagctccaag
      601 tcagatcatg aagaactttc aggctcctcc acaaatctcg ctgaccataa cccttcttct
      661 tggcgagacc acgatgatgc aacctcaacc cactcagcag gcaccccagg gccctccagt
      721 gggggccatg cttcccagag cggagacaac agcagtgagc aaggggatgg tttagacaac
      781 agtgtagctt cacctggtac aggtgacgat gatgatccgg ataaggacaa aaaacgccag
      841 aagaaaagag gcattttccc caaagtagca acaaatatca tgagagcatg gctcttccag
      901 catctcacac atccgtaccc ttccgaagag cagaagaaac agttagcgca agacacagga
      961 cttacaattc tccaagtaaa caactggttt attaatgcca gaagaagaat agtacagccc
     1021 atgattgacc agtcaaatcg agcaggtttt cttcttgatc cttcagtgag ccaaggagca
     1081 gcatatagtc cagagggtca gcccatgggg agctttgtgt tggatggtca gcaacacatg
     1141 gggatccggc ctgcaggttt gcagagcatg ccaggggact acgtttctca gggtggtcct
     1201 atgggaatga gtatggcaca gccaagttac actcctcccc agatgacccc acaccctact
     1261 caattaagac atggaccccc aatgcattca tatttgccaa gccatcccca ccacccagcc
     1321 atgatgatgc acggaggacc ccctacccac cctggaatga ctatgtcagc acagagcccc
     1381 acaatgttaa attctgtaga tcccaatgtt ggcggacagg ttatggacat tcatgcccaa
     1441 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1501 ggaaaggacg atacgtcacg ccgcagctcc tttct
//
