LOCUS       sequence-280814-        2339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1940
FEATURES             Location/Qualifiers
     source          1..2339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtgat gaacctgatg gagcagccta tcaaggtgac tgagtggcag
       61 cagacataca cctacgactc gggtatccac tcgggcgcca acacctgcgt gccctccgtc
      121 agcagcaagg gcatcatgga ggaggatgag gcctgcgggc gccagtacac gctcaagaaa
      181 accaccactt acacccaggg ggtgcccccc agccaaggtg atctggagta ccagatgtcc
      241 acaacagcca gggccaaacg ggtgcgggag gccatgtgcc ctggtgtgtc aggcgaggac
      301 agctcgcttc tgctggccac ccaggtggag gggcaggcca ccaacctgca gcgactggcc
      361 gagccgtccc agctgctcaa gtcggccatt gtgcatctca tcaactacca ggacgatgcc
      421 gagctggcca ctcgcgccct gcccgagctc accaaactgc tcaacgacga ggacccggtg
      481 gtggtgacca aggcggccat gattgtgaac cagctgtcga agaaggaggc gtcgcggcgg
      541 gccctgatgg gctcgcccca gctggtggcc gctgtcgtgc gtaccatgca gaataccagc
      601 gacctggaca cagcccgctg caccaccagc atcctgcaca acctctccca ccaccgggag
      661 gggctgctcg ccatcttcaa gtcgggtggc atccctgctc tggtccgcat gctcagctcc
      721 cctgtggagt cggtcctgtt ctatgccatc accacgctgc acaacctgct cctgtaccag
      781 gagggcgcca agatggccgt gcgcctggcc gacgggctgc aaaagatggt gcccctgctc
      841 aacaagaaca accccaagtt cctggccatc accaccgact gcctgcagct cctggcctac
      901 ggcaaccagg agagcaagct gatcatcctg gccaatggtg ggccccaggc cctcgtgcag
      961 atcatgcgta actacagtta tgaaaagctg ctctggacca ccagtcgtgt gctcaaggtg
     1021 ctatccgtgt gtcccagcaa taagcctgcc attgtggagg ctggtgggat gcaggccctg
     1081 ggcaagcacc tgaccagcaa cagcccccgc ctggtgcaga actgcctgtg gaccctgcgc
     1141 aacctctcag atgtggccac caagcaggag ggcctggaga gtgtgctgaa gattctggtg
     1201 aatcagctga gtgtggatga cgtcaacgtc ctcacctgtg ccacgggcac actctccaac
     1261 ctgacatgca acaacagcaa gaacaagacg ctggtgacac agaacagcgg tgtggaggct
     1321 ctcatccatg ccatcctgcg tgctggtgac aaggacgaca tcacggagcc tgccgtctgc
     1381 gctctgcgcc acctcactag ccgccaccct gaggccgaga tggcccagaa ctctgtgcgt
     1441 ctcaactatg gcatcccagc catcgtgaag ctgctcaacc agcccaacca gtggccactg
     1501 gtcaaggcaa ccatcggctt gatcaggaat ctggccctgt gcccagccaa ccatgccccg
     1561 ctgcaggagg cagcggtcat cccccgcctc gtccaactgc tggtgaaggc ccaccaggat
     1621 gcccagcgcc acgtagctgc aggcacacag cagccctaca cggatggtgt gaggatggag
     1681 gagattgtgg agggctgcac cggagcactg cacatcctcg cccgggaccc catgaaccgc
     1741 atggagatct tccggctcaa caccattccc ctgtttgtgc agctcctgta ctcgtcggtg
     1801 gagaacatcc agcgcgtggc tgccggggtg ctgtgtgagc tggcccagga caaggaggcg
     1861 gccgacgcca ttgatgcaga gggggcctcg gccccactca tggagttgct gcactcccgc
     1921 aacgagggca ctgccaccta cgctgctgcc gtcctgttcc gcatctccga ggacaagaac
     1981 ccagactacc ggaagcgcgt gtccgtggag ctcaccaact ccctcttcaa gcatgacccg
     2041 gctgcctggg aggctgccca gagcatgatt cccatcaatg agccctatgg agatgacatg
     2101 gatgccacct accgccccat gtactccagc gatgtgcccc ttgacccgct ggagatgcac
     2161 atggacatgg atggagacta ccccatcgac acctacagcg acggcctcag gcccccgtac
     2221 cccactgcag accacatgct ggcctagtaa tgaactagtc cgtaacttga aagtatttcg
     2281 atttcttggc tttatatatc ttgtggaaag gacgagtgca cctcgacatc tagttatac
//
