LOCUS       sequence-28082-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Tadeo X, Lopez-Mendez B, Trigueros T, Lain A, Castano D, Millet O
  TITLE     Structural basis for the aminoacid composition of proteins from 
            halophilic archea.
  JOURNAL   PLoS Biol. 2009 Dec;7(12):e1000257. doi: 
            10.1371/journal.pbio.1000257. Epub 2009 Dec 15.
  PUBMED    20016684
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2009-12"; volume: "7(12):e1000257. doi"; pages: " 
            10.1371/journal.pbio.1000257. Epub 2009 Dec 15"
COMMENT     Sequence Label: pDEST17_A1LigN_EC
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacat atgtcgtact accatcacca tcaccatcac
       61 ctcgaatcaa caagtttgta caaaaaagca ggcttcagcc agattcgcca tgaagtgcca
      121 atgctgtcgc tggataacgt ttttgatgaa gaaagctttc ttgccttcaa caaacgagtg
      181 caggaccgtc tgaaaagcaa cgagaaagtc acctggtgct gtgagctgaa gctggatggc
      241 cttgctgtca gtattctgta tgaaaatggc gttttagtca gtgccgcgac ccgtggcgat
      301 ggcaccaccg gggaagatat cacgtctaac gtgcgtacca ttcgcgccat tccactgaag
      361 ctacacggag agaatatccc ggcgcgtctg gaagtgcgtg gtgaagtgtt cctgccgcag
      421 gcggggttcg aaaagattaa cgaagatgcg cgacgcacgg gcgggaaagt ttttgctaac
      481 ccgcgtaatg cggcagctgg ttcactgcgt cagcttgatc cgcgtattac agcgaagcga
      541 ccgctcactt ttttctgcta tggcgttggt gttctggaag gtggcgagct gccggatact
      601 catcttggcc gtttactgca atttaaacag tgggggtttg ccggtcagcg atcgggtaac
      661 gctttgtgaa tctgcggaag aagtgctggc gttctatcac aaagtgnaag aagaccgccc
      721 cgacgctggg ctttgatatc gacggcgtgg tgattaaggt caactcactg gcacagcagg
      781 agcagcttgg cttttgtcgc gcgtgccccg cgctgggcgg tacgtttta
//
