LOCUS       sequence-280826-        1751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1951
FEATURES             Location/Qualifiers
     source          1..1751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggaggc ttcttttgga agttcgagcc cagttgggtc tttgtcttct
       61 gaggatcatg attttgaccc cactgctgag atgttggtcc atgactatga tgatgaaaga
      121 actcttgaag aagaggaaat gatggatgag ggtaaaaact tcagttcaga aattgaagac
      181 ttagaaaagg aaggaaccat gcctctagaa gatttactgg cattctatgg ctatgaacct
      241 acaattccag cagttgcaaa ttccagtgca aatagttccc caagtgaact ggcagatgaa
      301 ctaccagaca tgacactaga caaagaggaa atagcaaaag acctgttgtc aggtgatgac
      361 gaggaaactc agtcttctgc ggatgatctg acgccatctg tgacttccca tgaaacttct
      421 gatttcttcc ctaggccttt acgatcaaat actgcatgtg atggtgataa ggaatcagag
      481 gttgaagatg ttgaaacaga cagtggtaat tcacctgaag atttgaggaa ggaaataatg
      541 attggtttac aatatcaggc agagattccc ccttatcttg gagagtacga tggtaatgag
      601 aaagtatatg aaaacgaaga ccagttactt tggtgtcctg atgtggtttt ggagagcaaa
      661 gttaaggaat accttgttga gacttcatta aggactggca gtgaaaaaat aatggatagg
      721 atttctgcag gaacacacac aagggacaat gaacaggcat tatatgaact tctcaagtgt
      781 aaccacaata taaaggaagc aatcgaaaga tactgctgca atggaaaggc ctctcaagga
      841 atgactgcat ggacggaaga agaatgccga agctttgaac atgcactcat gctttttgga
      901 aaagattttc atcttataca gaagaataag gtgagaacta ggacagttgc tgagtgtgta
      961 gcattctact atatgtggaa gaaatctgaa cgttatgatt actttgctca acagacaaga
     1021 tttgggaaaa aaagatataa ccatcaccct ggagttacgg actatatgga tcgtttagta
     1081 gatgaaacag aagctttggg tgggacggta aatgcttcag ccttaacttc taaccggcct
     1141 gagcctattc ctgatcaaca gctaaacatt ctcaactcct tcactgccag tgacttgaca
     1201 gctttgacca acagtgtagc aaccgtctgc gaccccacag atgtgaattg tttggatgat
     1261 agctttcctc cactgggcaa cacaccccgt ggacaagtta atcatgtgcc tgttgtaaca
     1321 gaagagttac tcaccctgcc cagcaatggg gaaagtgatt gttttaattt atttgagact
     1381 ggattttatc actcggagct aaaccctatg aacatgtgca gtgaagagtc agagagacca
     1441 gcaaaaagat tgaaaatggg cattgccgtc cctgaatcct ttatgaatga agtttctgta
     1501 aataacctgg gtgtggactt tgaaaatcac acacatcaca tcaccagtgc caaaatggct
     1561 gtttctgtgg ctgactttgg cagtctctct gccaacgaga ccaatggttt catcagtgcc
     1621 catgctctgc atcagcacgc ggccctacac tctgagtagt aatgaactag tccgtaactt
     1681 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatct tatccgtgag
     1741 gtcggagcca a
//
